| Product Name | Creatinine Assay Reagent |
| Catalog No. | CMTR-HMM-0026 |
| Description | Creatinine, also known as creatinine, is a breakdown product of phosphocreatine in muscles. Creatine loses a water molecule to form creatinine. Creatinine is transported to the kidneys via plasma, then excreted from the body through glomerular filtration and partial tubular secretion. Creatinine is typically produced and excreted at a fairly constant rate, and its concentration in the blood can be used to measure glomerular filtration rate (GFR), an indicator of kidney function. |
| Application | This product is used to measure creatinine concentration in biological fluids such as serum, urine, or cerebrospinal fluid. |
| Principle | Creatinine is converted to creatine by creatine kinase, and creatine is converted to creatine by creatine kinase. Myosine is oxidized by myosin oxidase to produce hydrogen peroxide. Hydrogen peroxide is then processed by peroxidase, reacting with the probe to produce red color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). |
| Assay Type | Quantitative |
| Detection Method | Colorimetric; Fluorometric |
| Applicable Instruments | Microplate reader |
| Detection Time | 1 h |
| Sample Type | Other biological fluids, Serum, Urine |
| Procedure | 1. Add the sample and standard to the wells. 2. Add the reaction mixture and incubate at 37°C for 60 minutes. 3. Analyze using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
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