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| Product Name | Intracellular Oxygen Concentration Assay Reagent |
| Catalog No. | CMTR-HMM-0024 |
| Description | This is a method for detecting changes in oxygen concentration within cells through rapid dilution. |
| Application | This product is used to analyze intracellular oxygen concentration in cell monolayers. |
| Principle | This assay is based on the ability of oxygen to quench the excited state of an oxygen-sensitive probe. The probe is taken up by non-specific energy-dependent endocytosis, and after washing, cells are monitored on a fluorescence microplate reader (dual-reading TR-F is required for complete oxygen quantification). The probe's phosphorescence is quenched by intracellular oxygen in a non-chemical, reversible manner, enabling measurement of average intracellular O₂ levels and facilitating real-time monitoring of relative changes in cellular oxygen consumption. The probe signal intensifies as intracellular oxygen decreases and weakens as intracellular oxygen increases. The probe can be excited at 360–400 nm or 535 nm, with an emission wavelength of 630–680 nm. The optimal filter combination is Ex/Em = 340/642 nm. |
| Assay Type | Quantitative |
| Detection Method | Fluorescent |
| Applicable Instruments | Microplate reader |
| Detection Time | 1 h 30 min |
| Sample Type | Suspension cells, Adherent cells |
| Storage | 4°C |
For research use only, not for clinical use.
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