| Product Name | Glycogen Assay Reagent |
| Catalog No. | CMTR-HMM-0030 |
| Description | Glycogen is the primary short-term energy storage molecule in animals. It is primarily synthesized in the liver and muscles. Patients with diabetes and certain hereditary glycogen storage disorders exhibit abnormalities in glycogen utilization. |
| Application | This product is used to measure glycogen levels in biological samples. |
| Principle | Glycogen is hydrolyzed into glucose, which is oxidized to form an intermediate that reduces the colorless probe into a colored product with strong absorbance at 450 nm. |
| Assay Type | Quantitative |
| Detection Method | Colorimetric |
| Applicable Instruments | Microplate reader |
| Detection Time | 1 h |
| Sample Type | Adherent cells, Suspension cells, Tissue |
| Detection Limit | 4 µg/mL |
| Standard Linear Range | 4 µg/mL - 40 µg/mL |
| Procedure | 1. Add the sample and standard to the wells. 2. Add the hydrolase and incubate at room temperature for 30 minutes. 3. Add the reaction mixture and incubate at room temperature for 30 minutes. 4. Analyze using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
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