| Product Name | Lipid Peroxidation (MDA) Assay Reagent |
| Catalog No. | CMTR-HMM-0020 |
| Description | Lipid peroxidation refers to the oxidative degradation of lipids, which is a well-established mechanism of cellular damage. The formation of lipid peroxidation products leads to the propagation of free radical reactions, ultimately resulting in cellular damage. |
| Application | This product is used to detect malondialdehyde (MDA). |
| Principle | MDA in the sample reacts with thiobarbituric acid (TBA) to form MDA-TBA adducts. MDA-TBA adducts can be quantified using colorimetry (OD = 532 nm) or fluorescence (Ex/Em = 532/553 nm). |
| Synonyms | TBAR |
| Assay Type | Quantitative |
| Detection Method | Colorimetric; Fluorometric |
| Applicable Instruments | Microplate reader |
| Detection Time | 1 h 20 min |
| Sample Type | Cell culture extracts, Plasma, Urine, Tissue extracts |
| Detection Limit | 0.1 nmol/well |
| Procedure | 1. Add TBA solution to the sample and standard, incubate at 95°C for 60 minutes, and cool in an ice bath for 10 minutes. 2. Transfer to microplate wells. 3. Analyze using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
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