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| Product Name | Chemically Modified Hot-Start Taq DNA Polymerase |
| Catalog No. | IEC-HMM-0093 |
| Description | A chemically modified hot-start Taq DNA polymerase that remains fully inactive below 80°C and supports sensitive amplification of low-copy targets from complex templates. |
| Intended Use | Colony PCR; amplification of low-copy genes from genomic DNA or cDNA; hot-start PCR. |
| Principle / Technology | Chemical modification blocks polymerase activity below 80°C and is removed by a 95°C activation step. |
| Detection Method | Endpoint PCR or compatible qPCR formats. |
| Sample Type | Colonies, genomic DNA, cDNA and other complex DNA templates. |
| Performance Range / Specifications | 250, 1,000 or 3,000 U; complete activity release after approximately 5 minutes at 95°C. |
| Sensitivity / LOD | Optimized for low-copy amplification from complex templates. |
| Specificity | Complete low-temperature activity blocking reduces nonspecific amplification during setup. |
| Reaction Conditions / Protocol | Activate at 95°C for approximately 5 minutes before cycling, then use a target-appropriate PCR program. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 250 U; 1,000 U; 3,000 U. |
| Product Form | Chemically modified hot-start Taq DNA polymerase. |
| Key Features | Complete activity blocking below 80°C; 5-minute heat activation; high sensitivity; convenient hot-start PCR workflow. |
| Compatibility | Suitable for genomic DNA, cDNA and colony PCR templates. |
For research use only, not for clinical use.
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