| Product Name | Cell Senescence β-Galactosidase Staining Assay Reagent |
| Catalog No. | CATR-HMM-0012 |
| Description | Cell senescence refers to the process by which cells gradually lose their proliferative and differentiative capabilities and physiological functions over time during their life cycle. Under normal circumstances, the body clears senescent cells, but those not cleared may accumulate in the body, leading to low-level inflammation or further inducing the decline or carcinogenesis of adjacent tissues. Some characteristics of cell senescence include changes in cell morphology, cell cycle arrest, senescence-associated secretory phenotype, macromolecular damage, and metabolic disorders. Currently, the most specific marker for identifying cellular senescence is senescence-associated β-galactosidase (SA-β-Gal), which gradually accumulates as cells age but is absent in pre-senescent cells, quiescent cells, and terminally differentiated cells. |
| Application | This product utilizes β-Gal in senescent cells, which can catalyze the hydrolysis of 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) at a pH of 6.0, X-gal) at pH 6.0, which can be observed under an optical microscope to determine the senescence status of cells or tissues. |
| Applicable Instruments | Spectrophotometer, fluorescence microscope, flow cytometer |
| Materials to Bring | 96/24/12/6-well culture plates or culture dishes; 10 mM PBS, pH 7.2–7.6; 4% paraformaldehyde fixative (in PBS); permeabilization reagent (0.5% Triton X-100 in PBS); 2 mg/mL glycine solution (in ddH₂O); 3% BSA in PBS, pH 7.2–7.6; 1% BSA in PBS, pH 7.2–7.6; ddH₂O |
| Storage | Store at -20°C away from light. |
| Features | Obvious effect: the blue substance is clearly visible, making it easy to observe; Good stability: can withstand 20 cycles of repeated freezing and thawing and short-term transportation at room temperature. |
| Notes | 1. Before use, briefly centrifuge the product to the bottom of the tube, then proceed with subsequent experiments. 2. Cell senescence β-galactosidase detection relies on specific pH conditions, and the high CO₂ concentration in a CO₂ incubator can affect the pH of the staining solution. Therefore, incubation at 37°C should not be performed in a CO₂ incubator. 3. X-Gal solution may freeze when stored at -20°C or 4°C. To thaw, place the solution in a water bath at room temperature or 37°C for 2–5 minutes while gently shaking. If you need to aliquot the solution or prepare the staining working solution, use polypropylene (PP) or glass-based consumables. Do not use polystyrene (PS) consumables, such as cell culture plates or serum pipettes. 4. A small amount of precipitation may be present at the bottom of the β-galactosidase staining solution B tube, which is a normal phenomenon. Please vortex thoroughly until completely dissolved before proceeding with the staining experiment. 5. If staining of tissue sections is required, please refer to relevant literature and conduct preliminary experiments to optimize the protocol. 6. β-galactosidase staining fixative solution has certain toxicity and corrosiveness. When handling it, please wear a lab coat and disposable mask and gloves to avoid direct contact with the body or inhalation. |
For research use only, not for clinical use.
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