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| Product Name | Bst Plus DNA Polymerase (40 U/μL) |
| Catalog No. | IVDRM-0235 |
| Description | Bst Plus DNA Polymerase is derived from Thermophilic Geobacillus sp. DNA Polymerase I and genetically engineered to remove its 5'→3' exonuclease activity. The enzyme possesses strong 5'→3' DNA polymerase activity, strand displacement activity, and dUTP tolerance, making it ideal for contamination-controlled isothermal amplification reactions such as LAMP and CPA. |
| Intended Use | Raw material enzyme for isothermal amplification-based molecular diagnostics, including LAMP, CPA, and RCA (rolling circle amplification). |
| Principle / Technology | Engineered DNA Polymerase I from Thermophilic Geobacillus sp. lacking 5'→3' exonuclease activity. Strong 5'→3' polymerase and strand displacement activities enable DNA amplification at a constant temperature. |
| Performance Range / Specifications | - Suitable for multiple isothermal amplification methods, including LAMP, CPA, and RCA. - Supplied in a stabilized storage buffer (see Components / Formulation). - Heat inactivation: 85 °C for 5 min. |
| Reaction Conditions / Protocol | Unit activity is defined at 65 °C. Heat inactivate at 85 °C for 5 min after the reaction if required. |
| Components / Formulation | Enzyme storage buffer: 10 mM Tris-HCl, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol, pH 7.5 @ 25 °C. |
| Storage Conditions | Store at -25 °C to -15 °C. |
| Shelf Life | 2 years |
| Package Specifications | 8,000 U 40,000 U |
| Product Form | Liquid in storage buffer containing 50% glycerol |
| Concentration | 40 U/uL |
| Activity / Unit Definition | One unit (1 U) is defined as the amount of enzyme that incorporates 10 nmol of dNTPs into acid-insoluble material in 30 min at 65 °C. |
| Source / Origin | Recombinant, genetically engineered Thermophilic Geobacillus sp. DNA Polymerase I with the 5'→3' exonuclease activity removed. |
| Expiration Date / Stability | Stable for 2 years when stored at -25 °C to -15 °C. |
| Compatibility | Tolerates dUTP, making it compatible with dUTP/UDG carryover contamination control systems for isothermal amplification. |
| Application Notes / Precautions | - Recommended for LAMP, CPA, RCA, and other isothermal amplification reactions. - The strand displacement activity eliminates the need for thermal cycling. - dUTP tolerance allows incorporation of carryover contamination control strategies. |
| Heat Inactivation | 85 °C, 5 min |
For research use only, not for clinical use.
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