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| Product Name | Bst DNA Polymerase Large Fragment |
| Catalog No. | IEC-HMM-0112 |
| Description | A Bst DNA polymerase large fragment providing robust 5′→3′ polymerase and strand-displacement activities for isothermal amplification. |
| Intended Use | LAMP, rolling-circle amplification and other strand-displacement DNA amplification applications. |
| Principle / Technology | The large fragment derived from Bacillus stearothermophilus DNA polymerase retains 5′→3′ DNA synthesis and strong strand displacement while lacking 5′→3′ exonuclease activity. |
| Detection Method | Endpoint or real-time isothermal amplification, depending on the selected detection chemistry. |
| Sample Type | DNA templates used in isothermal amplification. |
| Performance Range / Specifications | 8,000 U; enzyme concentration 8 U/µL. |
| Reaction Conditions / Protocol | Use with the supplied 10× isothermal reaction buffer and magnesium sulfate in a validated LAMP or RCA workflow. |
| Components / Formulation | Bst DNA polymerase large fragment at 8 U/µL; 10× isothermal reaction buffer; 100 mM magnesium sulfate. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 8,000 U. |
| Product Form | Strand-displacing DNA polymerase enzyme system. |
| Key Features | Strong strand-displacement activity; suitable for LAMP and RCA; supplied with buffer and magnesium sulfate. |
| Concentration | 8 U/µL. |
| Source / Origin | Large fragment of Bacillus stearothermophilus DNA polymerase. |
| Recommended Buffer System | 10× buffer containing Tris-HCl, potassium chloride, ammonium sulfate, magnesium sulfate and nonionic detergent. |
| Application Notes / Precautions | Avoid opening completed LAMP reactions in the setup area because amplicon aerosols can cause contamination. |
For research use only, not for clinical use.
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