| Product Name | Bacterial/Fungal Cell Viability Assay Reagent (Chemiluminescence Method) |
| Catalog No. | CATR-HMM-0031 |
| Description | This product is a reagent that uses chemiluminescence to measure ATP levels within microbial cells, enabling ultra-high sensitivity and ultra-wide linear range quantitative detection of viable microbial cells in cultures. |
| Application | This product is widely used for detecting microbial contamination, microbial growth rates, and antimicrobial compound activity, screening antimicrobial compounds, and rapidly quantifying bacteria, fungi, and other microorganisms with higher sensitivity and a broader linear range. |
| Applicable Instruments | Enzyme-linked immunosorbent assay (ELISA) reader |
| Storage | Store at -80°C. |
| Shelf Life | 2 years |
| Features | This product has high detection sensitivity and a wide linear range. It exhibits good linearity for Escherichia coli (DH5α) and yeast (Y187) in the range of 100 to 1011 bacteria. This product is easy to use, provides stable readings, and has a fast detection speed, with testing completed in approximately 5-25 minutes. |
| Notes | Since luciferase activity is sensitive to temperature, both the microbial samples and the detection reagents must be equilibrated to room temperature before the reaction. The detection buffer can be dissolved and mixed at room temperature or in a water bath not exceeding 25°C, then mixed with the detection substrate to form the detection reagent. Microbial cultures in multiwell plates must also be thoroughly equilibrated to room temperature; otherwise, temperature gradients may exist between the central wells and peripheral wells of the plate, thereby affecting the intensity and stability of the fluorescent signal. Compounds with high solvent content may interfere with the luciferase reaction and thus affect the chemiluminescence signal. This interference can be excluded by setting up control wells containing microbial culture medium with solvents. Based on testing, DMSO content of 2% or less in the final assay system does not affect the reaction. Please use white or black 96-well plates or 384-well plates suitable for microbial culture. If transparent 96-well plates or 384-well plates are used, adjacent wells may interfere with each other during chemiluminescence detection. |
For research use only, not for clinical use.
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