| Product Name | ATP Luminescent Cell Viability Assay Reagent |
| Catalog No. | CATR-HMM-0001 |
| Description | ATP is the most important energy molecule within cells, serving as a measure of cellular metabolic activity and exhibiting a good linear correlation with the number of live cells. Therefore, ATP content can be used to indicate the number of live cells. |
| Application | This product utilizes the ATP-dependent luciferase-catalyzed luciferin luminescence reaction to measure intracellular ATP levels via chemiluminescence signals, thereby detecting cell viability or quantifying the number of live cells. |
| Applicable Instruments | Chemiluminescence analyzer, liquid scintillation counter |
| Materials to Bring | 1.5 mL centrifuge tube; ddH₂O; anti-fluorescence quencher |
| Storage | Store at -20°C away from light. |
| Features | The luminescence-based cell viability assay kit provided in this kit offers a wide linear range, high sensitivity, and excellent stability. In a 96-well plate, there is a good linear relationship within the range of 100 to 100,000 cells, but the upper limit for the number of cells that can be detected may vary depending on the cell type. Additionally, the kit is easy to use, with ready-to-use detection reagents that provide stable readings and fast detection speeds, completing the assay in approximately 10 minutes without the need for cell washing or changing/removing the culture medium. Compared to other common cell viability assay methods, such as Calcein-AM and CCK-8, the luminescence-based cell viability assay is simpler and faster. |
| Notes | 1. Before use, briefly centrifuge the product to the bottom of the tube, then proceed with subsequent experiments. 2. The reagent contains luciferase, and repeated freeze-thaw cycles may affect its activity. It is recommended to aliquot the reagent and store it at -20°C in the dark. 3. Both the reagent and cell samples must be equilibrated to room temperature before use to avoid affecting the enzymatic catalytic effect. 4. High drug concentrations may interfere with the luciferase reaction, thereby affecting the chemiluminescence signal. It is recommended to set up control wells with drug-containing cell culture medium to exclude solvent interference. |
For research use only, not for clinical use.
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