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| Product Name | ATP-Dependent Recombinant dsRNA Ligase for Modified siRNA Nick Sealing |
| Catalog No. | IEC-HMM-0186 |
| Description | Alta DiagnoTech double-stranded RNA ligase is a directed-evolution recombinant enzyme with enhanced ligation activity and no stated RNA endonuclease or exonuclease contamination. |
| Intended Use | For joining 3'-hydroxyl and 5'-phosphate termini at nicks between double-stranded RNA molecules, including modified siRNA fragments. |
| Principle / Technology | The enzyme consumes ATP to form an enzyme-AMP intermediate, transfers AMP to the 5'-phosphate at the nick and catalyzes formation of a 3'-5' phosphodiester bond. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 12 months |
| Package Specifications | 100 µL and 1 mL |
| Product Form | Liquid enzyme |
| Source / Origin | Recombinant expression in Escherichia coli |
| Alias | Double-Stranded RNA Ligase |
| Product Type | ATP-dependent dsRNA ligase |
| Function / Efficacy | Seals nicks in double-stranded RNA and supports ligation of differently modified siRNA sequences. |
For research use only, not for clinical use.
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