- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Anti-Taq DNA Polymerase Monoclonal Antibody (Exonuclease Blocking) |
| Catalog No. | IVDRM-0283 |
| Description | This monoclonal antibody is designed for hot-start PCR. It binds to Taq DNA polymerase and inhibits its 5' to 3' exonuclease activity at low temperatures. The antibody is denatured during the initial DNA denaturation step of PCR, restoring Taq DNA polymerase activity and thereby achieving a hot-start effect. Blocking the 5' to 3' exonuclease activity helps prevent probe degradation before amplification, making the antibody suitable for probe-based qPCR systems. No special antibody inactivation treatment is required, and the product can be used under conventional PCR reaction conditions. |
| Intended Use | Raw material for hot-start PCR/qPCR reagent formulation; used to block the 5' to 3' exonuclease activity of Taq DNA polymerase, preventing probe degradation in probe-based qPCR and enabling hot-start amplification. |
| Principle / Technology | Antibody-mediated hot-start mechanism: the monoclonal antibody binds Taq DNA polymerase and blocks its 5' to 3' exonuclease activity at low temperature. During the initial DNA denaturation step of PCR, the antibody is heat-denatured and dissociates, restoring enzyme activity for amplification. |
| Detection Method | Hot-start PCR / probe-based qPCR |
| Performance Range / Specifications | No mouse-derived genomic DNA contamination detected after 35 PCR cycles. After treatment at 94 °C for 30 s followed by reaction at 55 °C for 10 min, Taq DNA polymerase activity is restored to nearly 100%. |
| Specificity | High affinity for Taq DNA polymerase; the antibody still blocks Taq enzyme activity at 65 °C, effectively suppressing primer-dimer formation and nonspecific amplification. |
| Reaction Conditions / Protocol | At a concentration of 5 mg/mL, 1 uL of antibody is recommended to block 60-120 U of Taq enzyme exonuclease activity. Mix at room temperature for 1 hour (mix for 2 hours when the volume exceeds 200 mL; for larger volumes, customers should adjust the process accordingly), then store at -20 °C overnight before use. Because the specific activity of different Taq enzymes varies, the blocking ratio can be adjusted appropriately; a blocking efficiency above 95% is recommended. |
| Components / Formulation | Monoclonal antibody solution against the 5' to 3' exonuclease activity of Taq DNA polymerase, supplied at 5 mg/mL. |
| Storage Conditions | Store at -25 °C to -15 °C. |
| Shelf Life | 2 years |
| Package Specifications | 100 µg 1 mg 5 mg 10 mg 100 mg 1000 mg |
| Product Form | Liquid |
| Quality Control | Exonuclease residue test: 1 µg of product incubated with 0.5 µg of lambda DNA-HindIII at 37 °C for 4 h shows no change in DNA electrophoresis bands. Nickase residue test: 1 µg of product incubated with 0.5 µg of IL23R plasmid at 37 °C for 4 h shows no change in DNA electrophoresis bands. RNase residue test: 1 µg of product incubated with 0.5 µg of 293T cell total RNA at 37 °C for 4 h shows no change in RNA electrophoresis bands. No mouse genomic DNA contamination detected after 35 PCR cycles. |
| Concentration | 5 mg/mL |
| Activity / Unit Definition | 1 uL of antibody (5 mg/mL) blocks 60-120 U of Taq enzyme exonuclease activity; a blocking efficiency above 95% is recommended. |
| Source / Origin | Mouse monoclonal antibody against Taq DNA polymerase |
| Expiration Date / Stability | Stable for 2 years when stored at -25 °C to -15 °C. |
| Regulatory / Compliance | For use as an IVD raw material; for research and manufacturing use. |
| Compatibility | Compatible with conventional PCR reaction conditions; suitable for probe-based qPCR systems. No special antibody inactivation step is required. |
| Application Notes / Precautions | Because the specific activity of different Taq enzymes varies, the blocking ratio should be adjusted accordingly; a blocking efficiency above 95% is recommended. For volumes greater than 200 mL, extend mixing to 2 hours; for larger volumes, adjust the process as needed. Store the blocked enzyme at -20 °C overnight before use. |
| Recognition Site | Taq DNA polymerase (blocks 5' to 3' exonuclease activity) |
| Heat Inactivation | The antibody is fully inactivated after treatment at 94 °C for 30 s; after heat treatment followed by reaction at 55 °C for 10 min, Taq DNA polymerase activity is restored to nearly 100%. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
