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| Product Name | Advanced Reverse Transcriptase, Ultra-Clean (200 U/μL) |
| Catalog No. | IVDRM-0104 |
| Description | Advanced Reverse Transcriptase, Ultra-Clean (200 U/μL) is a next-generation reverse transcriptase developed through genetic engineering of an RNase H-deficient M-MLV reverse transcriptase. Compared with the parental enzyme, it delivers substantially faster synthesis and greater stability. A proprietary refinement and purification process reduces nuclease, host genomic DNA, and background bacterial gDNA residuals to extremely low levels, making the enzyme exceptionally well suited for the development of rapid, fully premixed RT-qPCR reagents and other contamination-sensitive molecular applications. |
| Intended Use | For RT-PCR and qPCR applications; particularly suited for the development of rapid, fully premixed RT-qPCR reagents and RNA-seq library preparation. |
| Principle / Technology | Genetically engineered derivative of M-MLV (H-) reverse transcriptase with greatly improved synthesis speed and stability; manufactured through an ultra-clean refinement process that minimizes nuclease and residual DNA contamination. |
| Sample Type | RNA templates for reverse transcription in RT-PCR/qPCR workflows. |
| Performance Range / Specifications | High sensitivity: outperforms a leading competing enzyme (competitor A) in Ct values and fluorescence signal amplitude across four targets. Excellent full-premix stability: after accelerated storage at 37°C for 5 days, amplification curve shape, fluorescence values, and Ct values remain unchanged. E. coli genomic DNA residue < 0.02 copies per 100 U. Excellent library preparation performance. A glycerol-free formulation is available on request. |
| Sensitivity / LOD | Lower Ct values and higher fluorescence amplitude than a leading competing enzyme across four tested targets. |
| Components / Formulation | Supplied as a standalone enzyme at 200 U/μL; a glycerol-free version is available. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 1 year when stored at -25°C to -15°C. |
| Package Specifications | 10 KU; 100 KU; 200 KU |
| Quality Control | Ultra-low residuals of nucleases, host genomic DNA, and background bacterial genomic DNA; E. coli genomic DNA residue verified at < 0.02 copies per 100 U. Performance stability confirmed after 37°C accelerated storage for 5 days and after lyophilization. |
| Purity | Ultra-clean grade; extremely low nuclease and residual DNA contamination. |
| Concentration | 200 U/μL |
| Source / Origin | Genetically engineered from M-MLV (H-) reverse transcriptase. |
| Expiration Date / Stability | Stable for 1 year at -25°C to -15°C; full-premix formulations remain stable after 5-day accelerated storage at 37°C and retain performance after lyophilization. |
| Recommended Buffer System | Optimized reverse transcription buffer for RT-PCR/qPCR applications. |
| Batch-to-Batch Consistency | Manufactured under a tightly controlled ultra-clean process to ensure consistent performance between lots. |
For research use only, not for clinical use.
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