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| Product Name | Advanced Reverse Transcriptase, Ultra-Clean (200 U/μL, Glycerol-Free) |
| Catalog No. | IVDRM-0241 |
| Description | This enzyme is a next-generation reverse transcriptase obtained by genetic engineering of an RNase H-deficient M-MLV reverse transcriptase. Compared with the parental enzyme, it offers substantially higher synthesis speed and stability. A dedicated refining process delivers an ultra-clean, low nucleic acid background product with extremely low residual nucleases, host genomic DNA, and background bacterial genomic DNA, making it well suited for the development of rapid full-premix RT-qPCR reagents. The formulation contains no glycerol and can be used to prepare lyophilized RT-qPCR reagents; data show that the lyophilized reagents retain high detection sensitivity and excellent stability. |
| Intended Use | Raw material for IVD molecular diagnostic reagent development, especially rapid full-premix RT-qPCR reagents and lyophilized RT-qPCR formulations. |
| Principle / Technology | Genetically engineered M-MLV (H-) reverse transcriptase with RNA-dependent DNA polymerase activity and deficient RNase H activity; manufactured by a dedicated refining process to achieve ultra-low nucleic acid background. |
| Detection Method | RT-qPCR / RT-PCR |
| Sample Type | RNA templates (e.g., total RNA) |
| Performance Range / Specifications | High sensitivity: lower Ct values and higher fluorescence amplitude across four tested targets than comparable market alternatives Excellent full-premix stability: after accelerated storage at 37 °C for 5 days, amplification curve shape, fluorescence values, and Ct values remain unchanged E. coli genomic DNA residue < 0.02 copies/100 U Excellent library preparation performance Stable performance after lyophilization Glycerol-free formulation |
| Sensitivity / LOD | E. coli genomic DNA residue < 0.02 copies/100 U |
| Specificity | Ultra-clean, low nucleic acid background; extremely low residual nucleases, host gDNA, and background bacterial gDNA |
| Components / Formulation | Glycerol-free liquid enzyme formulation, 200 U/μL |
| Storage Conditions | Store at -25 to -15 °C. |
| Shelf Life | 1 year |
| Package Specifications | 10 KU 100 KU 200 KU |
| Product Form | Glycerol-free liquid |
| Quality Control | Residual E. coli genomic DNA < 0.02 copies/100 U; ultra-low residual nucleases and host/background bacterial genomic DNA; performance verified after lyophilization and 37 °C accelerated stability testing |
| Purity | Ultra-clean grade with extremely low nucleic acid background |
| Concentration | 200 U/μL |
| Source / Origin | Genetically engineered RNase H-deficient M-MLV (H-) reverse transcriptase, recombinantly expressed |
| Expiration Date / Stability | Valid for 1 year when stored at -25 to -15 °C; full-premix reagents remain stable after 5 days of accelerated storage at 37 °C |
| Compatibility | Suitable for full-premix RT-qPCR reagent development and lyophilized (freeze-dried) RT-qPCR formulations |
| Application Notes / Precautions | The glycerol-free formulation is designed for lyophilized reagent manufacturing; avoid repeated freeze-thaw cycles. |
For research use only, not for clinical use.
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