Acyl-CoA Oxidase for Free Fatty Acid NEFA Detection >=10 U/mg 70 kDa Isoelectric Point 6.2 CAS 61116-22-1

Name: Acyl-CoA Oxidase for Free Fatty Acid NEFA Detection >=10 U/mg 70 kDa Isoelectric Point 6.2 CAS 61116-22-1
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Cat.No: IEC-HMM-0277 Datasheet

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Product Name	Acyl-CoA Oxidase for Free Fatty Acid NEFA Detection >=10 U/mg 70 kDa Isoelectric Point 6.2 CAS 61116-22-1
Catalog No.	IEC-HMM-0277
Description	Acyl-CoA oxidase offered by Alta DiagnoTech catalyzes the first step of fatty acid beta-oxidation in peroxisomes. It is an essential component of non-esterified fatty acid (NEFA) clinical chemistry reagents, producing hydrogen peroxide for quantitative detection.
Intended Use	Core raw enzyme for NEFA assay kits and fatty acid metabolism research.
Principle / Technology	Oxidizes acyl-CoA to trans-2,3-dehydroacyl-CoA with concomitant reduction of O2 to H2O2, which is then quantified by a Trinder-type chromogenic reaction.
Detection Method	Colorimetric endpoint assay via hydrogen peroxide detection.
Sample Type	Serum, plasma.
Performance Range / Specifications	Specific activity >=10 U/mg. Molecular weight approximately 70 kDa. Isoelectric point 6.2.
Components / Formulation	Acyl-CoA oxidase lyophilized powder.
Storage Conditions	-20°C.
Shelf Life	Stable for at least 12 months at -20°C.
Package Specifications	KU packaging units.
Product Form	Pale yellow amorphous powder.
Purity	99%
Activity / Unit Definition	>=10 U/mg
Molecular Weight	Approximately 70 kDa
Source / Origin	Microbial fermentation.
pH Range / Optimal pH	Optimal pH 7.0-9.0; stable between pH 6.0-8.5 at 37°C for 20 hours.
Shipping Conditions	Cold chain shipment.
Expiration Date / Stability	-20°C stable for one year.
Appearance	Pale yellow amorphous powder.
CAS Number	61116-22-1
Isoelectric Point	6.2
Inhibitors	Not inhibited by sodium azide.
Optimal Temperature	35-50°C.
Thermal Stability	Stable below 45°C for 15 minutes.
Handling Precaution	Protect from moisture and repeated freeze-thaw cycles.

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For research use only, not for clinical use.