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| Product Name | Acyl-CoA Oxidase (ACO) |
| Catalog No. | IEC-HMM-0125 |
| Description | Alta DiagnoTech Acyl-CoA Oxidase (ACO) is a recombinant enzyme supplied as yellow lyophilized powder. The enzyme has a molecular weight of 77 kDa (SDS-PAGE) by SDS-PAGE. |
| Intended Use | For free fatty acid determination in blood. |
| Principle / Technology | One unit converts 1 µmol of acyl-CoA to trans-2,3-dehydroacyl-CoA per minute at pH 7.5 and 37°C. |
| Sample Type | Blood samples in free fatty acid assay workflows |
| Performance Range / Specifications | EC 1.3.3.6; CAS 61116-22-1; Specific activity: ≥30 U/mg protein |
| Reaction Conditions / Protocol | Optimum pH: 8.5; Optimum temperature: 37-40°C; pH stability: pH 6.0-8.5 at 25°C for 15 hours; Thermal stability: Below 45°C at pH 7.5 for 15 minutes; Inhibitors: Ag+, Hg2+, Zn2+, Cu2+, and Ni2+ |
| Components / Formulation | Yellow lyophilized powder |
| Storage Conditions | Store at or below -20°C. |
| Shelf Life | 18 months at -20°C. |
| Product Form | Yellow lyophilized powder |
| Key Features | Yellow lyophilized powder; Molecular weight: 77 kDa (SDS-PAGE); EC 1.3.3.6; Specific activity: ≥30 U/mg protein; Optimum pH: 8.5 |
| Activity / Unit Definition | Specific activity: ≥30 U/mg protein; Unit definition: One unit converts 1 µmol of acyl-CoA to trans-2,3-dehydroacyl-CoA per minute at pH 7.5 and 37°C. |
| Molecular Weight | 77 kDa (SDS-PAGE) |
| Source / Origin | Recombinant |
| pH Range / Optimal pH | 8.5 |
| Expiration Date / Stability | pH 6.0-8.5 at 25°C for 15 hours; Below 45°C at pH 7.5 for 15 minutes |
| Alternative Names / Synonyms | Acyl-CoA Oxidase (ACO); ACO |
For research use only, not for clinical use.
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