80 U/µL Glycerol-Free Murine RNase Inhibitor for Low-DTT Systems
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80 U/µL Glycerol-Free Murine RNase Inhibitor for Low-DTT Systems

Cat.No: IEC-HMM-0209 Datasheet

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Product Name 80 U/µL Glycerol-Free Murine RNase Inhibitor for Low-DTT Systems
Catalog No. IEC-HMM-0209
Description Alta DiagnoTech glycerol-free recombinant murine RNase inhibitor protects RNA by binding RNase A, B and C.
Intended Use For RNA protection in RT-PCR, RT-qPCR, in vitro-transcribed mRNA and glycerol-free or lyophilized workflows.
Principle / Technology Noncovalent 1:1 binding inhibits RNase A, B and C. The murine inhibitor remains stable at DTT concentrations below 1 mM.
Performance Range / Specifications Active from 25°C to 55°C and across pH 5-9, with maximum activity at pH 7-8; inactivated at 65°C or above. Does not inhibit RNase H, RNase 1 or RNase T1.
Storage Conditions Store at -25°C to -15°C and limit freeze-thaw exposure to five cycles.
Shelf Life 1 year
Package Specifications Available as 2 kU/25 µL, 20 kU/250 µL and 200 kU/2,500 µL formats.
Product Form Glycerol-free liquid enzyme inhibitor
Concentration 80 U/µL
Source / Origin Recombinant murine protein expressed in Escherichia coli
Compatibility Compatible with commercial reverse transcriptases, DNA polymerases and RNA polymerases.
Application Notes / Precautions Do not vortex or stir vigorously because strong agitation can inactivate the inhibitor.
Alias Glycerol-free RNase inhibitor
CAS Number 9001-99-4
Product Type Glycerol-free recombinant murine RNase inhibitor
Function / Efficacy Protects RNA from degradation by RNase A, B and C.

For research use only, not for clinical use.

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