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| Product Name | >=8 U/mg Cholesterol Oxidase for HDL-C and LDL-C Reagents |
| Catalog No. | IEC-HMM-0166 |
| Description | Alta DiagnoTech cholesterol oxidase is a yellow amorphous lyophilized enzyme powder for hydrogen-peroxide-generating lipoprotein cholesterol assays. |
| Intended Use | Enzyme raw material for research and bulk formulation of high- and low-density lipoprotein cholesterol reagents. |
| Performance Range / Specifications | Specific activity >=8 U/mg dry powder; purity >=90.0% by SDS-PAGE; catalase <=0.001%; cholesterol esterase <=0.001%; glucose oxidase <=0.01%; ATPase <=0.005%. |
| Storage Conditions | Store at -25 to -15 degrees C |
| Shelf Life | Maintains at least 90% activity for 12 months at -25 to -15 degrees C |
| Package Specifications | 2 kU, 100 kU, 500 kU or 1,000 kU |
| Product Form | Yellow amorphous lyophilized powder |
| Purity | >=90.0% by SDS-PAGE |
| Activity / Unit Definition | One unit is the amount of enzyme that generates 1 umol hydrogen peroxide per minute under the stated conditions. |
| Molecular Weight | 60 kDa by SDS-PAGE |
| Source / Origin | Microbial source |
| pH Range / Optimal pH | Optimum pH 7.0; stable at pH 5.5-10.0 for 16 hours at 25 degrees C |
| Appearance | Yellow amorphous lyophilized powder |
| Alias | Cholesterol Oxidase |
| Product Type | Cholesterol oxidase enzyme raw material |
| Function / Efficacy | Oxidize cholesterol and generate hydrogen peroxide in lipoprotein cholesterol assays. |
| Abbreviation | CO |
| EC Number | EC 1.1.3.6 |
| Specific Activity | >=8 U/mg dry powder |
| Isoelectric Point | 5.6 |
| Inhibitors | SDS, Ag+, Zn2+ and Hg2+ |
| Optimal Temperature | 50 degrees C |
| Thermal Stability | Stable below 40 degrees C at pH 7.0 for 30 minutes |
| Michaelis Constant | 3.3 x 10^-4 M for cholesterol |
| Stabilizer | BSA and nonionic surfactants |
For research use only, not for clinical use.
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