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| Product Name | ≥600 U/mg Microbial Diaphorase for β-Hydroxybutyrate Reagents |
| Catalog No. | IEC-HMM-0180 |
| Description | Alta DiagnoTech microbial diaphorase is a yellow lyophilized enzyme powder intended for β-hydroxybutyrate reagent development and scale-up. |
| Intended Use | For development and large-scale preparation of β-hydroxybutyrate reagents. |
| Storage Conditions | Store undisturbed at -25°C to -15°C. |
| Product Form | Lyophilized enzyme powder |
| Purity | ≥90.0% by SDS-PAGE |
| Activity / Unit Definition | One unit is the amount of enzyme that consumes 1 µmol DCPIP per minute under the stated reaction conditions. |
| Molecular Weight | 28 kDa by SDS-PAGE |
| Source / Origin | Microbial |
| pH Range / Optimal pH | Optimal pH 7.5; stable at pH 4.5-9.0 for 16 hours at 25°C |
| Expiration Date / Stability | Retains more than 90% activity for 12 months at -25°C to -15°C. |
| Appearance | Yellow amorphous lyophilized powder |
| Alias | Diaphorase |
| CAS Number | 9001-18-7 |
| Product Type | Diaphorase raw material |
| Function / Efficacy | Supports redox-coupled β-hydroxybutyrate assays. |
| Abbreviation | DPH |
| EC Number | 1.6.5.2 |
| Specific Activity | ≥600.0 U/mg enzyme powder |
| Isoelectric Point | 5.2 |
| Inhibitors | Fe3+, Mn2+ and Cu2+ |
| Optimal Temperature | 45°C |
| Thermal Stability | Stable below 50°C for 30 minutes at pH 7.5 |
| Michaelis Constant | 7.4 × 10^-4 M for NADH; 6.0 × 10^-2 M for NADPH |
| Stabilizer | FMN and NAD(P)H |
| Interference | Glucose-6-phosphate dehydrogenase ≤0.01%; NAD(P)H oxidase ≤0.01%. |
For research use only, not for clinical use.
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