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| Product Name | >=5.8 U/mg Creatine Amidinohydrolase for Enzymatic Creatinine Reagents |
| Catalog No. | IEC-HMM-0167 |
| Description | Alta DiagnoTech creatine amidinohydrolase is a white amorphous lyophilized enzyme powder that converts creatine to sarcosine in enzymatic creatinine systems. |
| Intended Use | Enzyme raw material for research and bulk formulation of enzymatic creatinine reagents. |
| Performance Range / Specifications | Specific activity >=5.8 U/mg dry powder; protein purity >=90%; catalase <=0.03%; creatinine amidohydrolase <=0.01%; sarcosine oxidase <=0.01%; NADH oxidase <=0.01%. |
| Package Specifications | 5 kU, 100 kU, 500 kU or 1,000 kU |
| Product Form | White amorphous lyophilized powder |
| Purity | >=90% protein purity |
| Activity / Unit Definition | One unit is the amount of enzyme required to completely convert 1 umol creatine to sarcosine per minute under the stated conditions. |
| Molecular Weight | 48 kDa by SDS-PAGE |
| Source / Origin | Microbial source |
| pH Range / Optimal pH | Optimum pH 8.0; stable at pH 4.5-10.0 for 16 hours at 25 degrees C |
| Appearance | White amorphous lyophilized powder |
| Alias | Creatine Amidinohydrolase |
| Product Type | Creatine amidinohydrolase enzyme raw material |
| Function / Efficacy | Hydrolyze creatine to sarcosine in coupled creatinine assays. |
| Abbreviation | CR |
| EC Number | EC 3.5.3.3 |
| Specific Activity | >=5.8 U/mg dry powder |
| Isoelectric Point | 4.6 |
| Inhibitors | Ag+, Cu2+ and Hg2+ |
| Optimal Temperature | 45 degrees C |
| Thermal Stability | Stable below 50 degrees C at pH 7.5 for 30 minutes |
| Michaelis Constant | 8.81 x 10^-3 M for creatinine |
| Stabilizer | EDTA and sugars |
For research use only, not for clinical use.
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