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| Product Name | >=55 U/mg 3-Alpha-Hydroxysteroid Dehydrogenase for TBA Reagents |
| Catalog No. | IEC-HMM-0137 |
| Description | Alta DiagnoTech 3-alpha-hydroxysteroid dehydrogenase is a white amorphous lyophilized enzyme powder for NADH-generating total bile acid reagent systems. |
| Intended Use | Enzyme raw material for research and bulk formulation of total bile acid reagents. |
| Performance Range / Specifications | Specific activity >=55.0 U/mg dry powder; purity >=90.0% by SDS-PAGE; glucose-6-phosphate dehydrogenase <=0.01%; lactate dehydrogenase <=0.005%; glutamate dehydrogenase <=0.001%; malate dehydrogenase <=0.01%; hexokinase <=0.01%. |
| Storage Conditions | Store at -25 to -15 degrees C |
| Shelf Life | Maintains at least 90% activity for 12 months at -25 to -15 degrees C |
| Package Specifications | 3 kU, 100 kU, 500 kU or 1,000 kU |
| Product Form | White amorphous lyophilized powder |
| Purity | >=90.0% by SDS-PAGE |
| Activity / Unit Definition | One unit is the amount of enzyme that generates 1 umol NADH per minute under the stated conditions. |
| Molecular Weight | 30 kDa by SDS-PAGE |
| Source / Origin | Microbial source |
| pH Range / Optimal pH | Optimum pH 8.5-9.5; stable at pH 6.0-9.0 for 16 hours at 25 degrees C |
| Appearance | White amorphous lyophilized powder |
| Alias | 3-Alpha-Hydroxysteroid Dehydrogenase |
| Product Type | 3-alpha-hydroxysteroid dehydrogenase enzyme raw material |
| Function / Efficacy | Generate NADH in enzymatic total bile acid reagent systems. |
| Abbreviation | 3-alpha-HSD |
| EC Number | EC 1.1.1.50 |
| Specific Activity | >=55.0 U/mg dry powder |
| Isoelectric Point | 5.2 |
| Inhibitors | Cu2+, Ag+, Hg2+, Zn2+ and Fe3+ |
| Optimal Temperature | 55 degrees C |
| Michaelis Constant | 3.6 x 10^-5 M for androsterone; 4.7 x 10^-5 M for NAD+ |
| Stabilizer | BSA |
For research use only, not for clinical use.
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