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| Product Name | >5.0 U/mg Recombinant PEPC for Carbon Dioxide Detection Reagents |
| Catalog No. | IEC-HMM-0239 |
| Description | Alta DiagnoTech phosphoenolpyruvate carboxylase is a white amorphous lyophilized enzyme powder designed for carbon-dioxide detection reagent development. |
| Intended Use | Used with malate dehydrogenase for carbon-dioxide detection reagent research and bulk formulation. |
| Performance Range / Specifications | Dry-powder specific activity >5.0 U/mg; pyruvate kinase contamination <=0.05%; lactate dehydrogenase contamination <=0.001%. |
| Storage Conditions | Store at -25 to -15 degrees C |
| Product Form | White amorphous lyophilized powder |
| Molecular Weight | 104.4 kDa by SDS-PAGE |
| Source / Origin | Recombinantly expressed from a microbial source |
| pH Range / Optimal pH | Optimum pH 5.0-9.0; stable at pH 5.5-9.0 for 24 hours at 25 degrees C |
| Recommended Buffer System | Optimum-pH testing: 50 mM sodium acetate/acetic acid at pH 4.0-5.5, potassium phosphate at pH 6.0-8.0, or Tris-HCl at pH 7.5-9.0. Temperature testing uses 20 mM potassium phosphate at pH 7.0. Stability studies use 1 mg/mL protein. |
| Appearance | White amorphous lyophilized powder |
| Other Name | Phosphoenolpyruvate Carboxylase |
| Product Type | Phosphoenolpyruvate carboxylase enzyme raw material |
| Function / Efficacy | Provide PEPC activity in coupled carbon-dioxide detection systems. |
| Abbreviation | PEPC |
| EC Number | EC 4.1.1.31 |
| Specific Activity | >5.0 U/mg dry powder |
| Isoelectric Point | 6.0 +/- 0.1 |
| Optimal Temperature | 60 degrees C |
| Thermal Stability | Stable below 45 degrees C at pH 7.0 for 30 minutes |
| Stabilizer | BSA and sugars |
For research use only, not for clinical use.
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