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| Product Name | >=50 U/mg Glucose Dehydrogenase for NADP-Coupled Glucose Assays |
| Catalog No. | IEC-HMM-0193 |
| Description | Alta DiagnoTech glucose dehydrogenase is a white amorphous powder for blood-glucose test-strip raw materials and NADP-coupled glucose measurement. |
| Intended Use | Enzyme raw material for blood glucose test strips and glucose assay development. |
| Detection Method | Kinetic absorbance measurement of NADH formation at 340 nm |
| Performance Range / Specifications | Specific activity >=50 U/mg; assay linear range 100-1,000 U/L; recommended sample range 200-700 U/L. |
| Reaction Conditions / Protocol | Prepare Reagent 1 by dissolving 1.211 g Tris and 26 g glucose in 50 mL deionized water, adjust to pH 7.55 at 25 degrees C with HCl and bring to 100 mL. Prepare Reagent 2 by dissolving 2.0 g NADP disodium salt in 40 mL water and bringing to 50 mL. Combine 1.00 mL Reagent 1 with 0.040 mL enzyme solution, preincubate for 60 seconds at 37 degrees C, add 0.200 mL Reagent 2, react for 1 minute and measure the 1-minute absorbance-change rate at 340 nm. Activity in U/L equals Delta A/min x 4,980 x dilution factor. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | At least 1 year at -20 degrees C |
| Product Form | White amorphous powder |
| Activity / Unit Definition | One unit is the amount of enzyme that generates 1 umol NADH per minute under the stated reaction conditions. |
| Molecular Weight | 28 kDa |
| pH Range / Optimal pH | Stable at pH 5.0-9.5; optimum pH is above 9.0 |
| Recommended Buffer System | Sample diluent: dissolve 12.1 g Tris and 1 g BSA in 800 mL deionized water, adjust to pH 8.0 at 25 degrees C with HCl and bring to 1 L. |
| Appearance | White amorphous powder |
| Alias | Glucose Dehydrogenase |
| Product Type | Glucose dehydrogenase enzyme raw material |
| Function / Efficacy | Oxidize glucose while reducing NAD or NADP for coupled glucose measurement. |
| Abbreviation | GDH |
| Customization | Specifications can be customized |
| Specific Activity | >=50 U/mg |
| Isoelectric Point | 5.16 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | >60 degrees C |
| Thermal Stability | Stable below 70 degrees C |
| Michaelis Constant | 0.8 x 10^-2 mol/L for glucose; 1.5 x 10^-4 mol/L for NAD; 4.3 x 10^-5 mol/L for NADP |
| Detection Wavelength | 340 nm |
| Linear Range | 100-1,000 U/L |
For research use only, not for clinical use.
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