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| Product Name | >=5 U/mg N-Acetylneuraminic Acid Aldolase for Sialic Acid Assays |
| Catalog No. | IEC-HMM-0236 |
| Description | Alta DiagnoTech N-acetylneuraminic acid aldolase is a white amorphous enzyme powder for sialic acid reagent development. |
| Intended Use | Enzyme raw material for biochemical sialic acid assay kits. |
| Detection Method | Lactate-dehydrogenase-coupled NADH assay at 340 nm |
| Performance Range / Specifications | Specific activity >=5 U/mg; assay linear range 100-300 U/L. |
| Reaction Conditions / Protocol | Prepare 50 mM potassium phosphate at pH 7.5 with 50 mM N-acetylneuraminic acid, 50 U/mL L-lactate dehydrogenase and 1.0 mM NADH. Preincubate 1.0 mL substrate, 0.5 mL LDH, 0.5 mL NADH and 0.4 mL buffer for 5 minutes at 37 degrees C, add 0.1 mL sample and calculate the 180-240 second rate at 340 nm against a buffer blank. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | At least 6 months at -20 degrees C |
| Product Form | White amorphous powder |
| Activity / Unit Definition | One unit cleaves 1 umol N-acetylneuraminic acid per minute under the stated assay conditions. |
| Molecular Weight | 32.6 kDa |
| pH Range / Optimal pH | Stable at pH 5.5-10.0 for 24 hours at 25 degrees C; optimum pH 6.5-8.0 |
| Appearance | White amorphous powder |
| Alias | N-Acetylneuraminic acid aldolase |
| Product Type | N-acetylneuraminic acid aldolase enzyme raw material |
| Function / Efficacy | Cleave N-acetylneuraminic acid for coupled sialic acid measurement. |
| Abbreviation | NAL |
| Customization | Specifications can be customized |
| Specific Activity | >=5 U/mg |
| Isoelectric Point | 5.45 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | >=55 degrees C |
| Thermal Stability | No activity loss at or below 70 degrees C for 30 minutes |
| Michaelis Constant | 5.7 x 10^-3 M for N-acetylneuraminic acid |
| Detection Wavelength | 340 nm |
| Linear Range | 100-300 U/L |
For research use only, not for clinical use.
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