- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | 5 U/µL Exonuclease-Deficient Hot-Start Taq for Forensic Multiplex PCR |
| Catalog No. | IEC-HMM-0305 |
| Description | Alta DiagnoTech antibody-modified hot-start Taq polymerase lacks 5'-to-3' exonuclease activity and remains strongly blocked below 70°C. |
| Intended Use | For multiplex PCR, including forensic amplification workflows and primer/probe premix formulations. |
| Principle / Technology | Site-directed mutation removes 5'-to-3' exonuclease activity, while a specific antibody blocks polymerase activity below 70°C. Heating at 95°C for 30 seconds releases more than 95% of enzyme activity. |
| Performance Range / Specifications | Maintains strong activity blockade below 70°C and releases more than 95% activity after 30 seconds at 95°C. |
| Reaction Conditions / Protocol | Activate at 95°C for 30 seconds. A final concentration of 0.2 µM per primer is recommended as a starting point. |
| Components / Formulation | The enzyme is supplied at 5 U/µL with a 5× Taq buffer. The 1 kU, 10 kU and 100 kU formats contain 200 µL, 2 mL and 20 mL enzyme, respectively, with 1 mL, 10 mL and 100 mL buffer. |
| Storage Conditions | Store at -25°C to -15°C. |
| Package Specifications | Available in 1 kU, 10 kU and 100 kU formats. |
| Concentration | 5 U/µL |
| Activity / Unit Definition | One unit incorporates 10 nmol of deoxynucleotide into DNA in 30 minutes at 72°C using M13 mp18 single-stranded virion DNA as template-primer. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | Designed for multiplex PCR and forensic testing. |
| Application Notes / Precautions | Validate each primer by PCR and calibrate the actual concentration of newly synthesized primers. Increase the corresponding primer amount moderately if a target produces low yield. |
| Product Type | Exonuclease-deficient antibody-modified hot-start Taq DNA polymerase |
| Function / Efficacy | Reduces nonspecific amplification and improves primer/probe premix stability, specificity and low-template sensitivity. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
