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| Product Name | 5 U/µL Dual-Blocked Hot-Start Taq with 30-Second Activation |
| Catalog No. | IEC-HMM-0304 |
| Description | Alta DiagnoTech dual-blocked hot-start Taq DNA polymerase uses antibody control of both polymerase and 5'-to-3' exonuclease activities at room temperature. |
| Intended Use | For fast singleplex or multiplex quantitative PCR using DNA templates, including primer/probe premix systems. |
| Principle / Technology | Antibody modification suppresses polymerase and exonuclease activities during setup, reducing mismatch extension, primer dimers and premature primer or probe degradation. Heating at 95°C for 30 seconds releases full enzyme activity. |
| Reaction Conditions / Protocol | Activate at 95°C for 30 seconds. |
| Components / Formulation | The enzyme is supplied at 5 U/µL with a 5× Taq buffer. The 1 kU, 10 kU and 100 kU formats contain 200 µL, 2 mL and 20 mL enzyme, respectively, with 2 × 1 mL, 2 × 10 mL and 2 × 100 mL buffer. |
| Storage Conditions | Store at -25°C to -15°C. |
| Package Specifications | Available in 1 kU, 10 kU and 100 kU formats. |
| Concentration | 5 U/µL |
| Activity / Unit Definition | One unit incorporates 10 nmol of deoxynucleotide into DNA in 30 minutes at 72°C using M13 mp18 single-stranded virion DNA as template-primer. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | Compatible with fast PCR programs and primer/probe premixing. |
| Application Notes / Precautions | Avoid repeated freeze-thaw cycles and wear gloves during handling. |
| Product Type | Antibody-modified hot-start Taq DNA polymerase |
| Function / Efficacy | Supports sensitive and specific rapid DNA amplification while protecting premixed primers and probes before activation. |
For research use only, not for clinical use.
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