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| Product Name | >=45 U/mg Lactate Oxidase for 555 nm Lactate Detection |
| Catalog No. | IEC-HMM-0224 |
| Description | Alta DiagnoTech lactate oxidase is a yellow amorphous lyophilized enzyme powder for quantitative lactate detection. |
| Intended Use | Enzyme raw material for lactate-content measurement. |
| Principle / Technology | The generated quinoneimine dye is monitored spectrophotometrically at 555 nm. |
| Detection Method | Coupled spectrophotometric assay at 555 nm |
| Performance Range / Specifications | Dry-powder specific activity >=45.0 U/mg; purity >=90.0% by SDS-PAGE; pyruvate oxidase, choline oxidase, uricase and glucose oxidase each <=0.001%. |
| Storage Conditions | Store at -25 to -15 degrees C |
| Shelf Life | Maintains at least 90% activity for 12 months at -25 to -15 degrees C |
| Package Specifications | 3 kU, 100 kU, 500 kU, 1,000 kU or custom packaging |
| Product Form | Yellow amorphous lyophilized powder |
| Purity | >=90.0% by SDS-PAGE |
| Activity / Unit Definition | One unit is the amount of enzyme that generates 1 umol hydrogen peroxide per minute under the stated assay conditions. |
| Molecular Weight | 42 kDa by SDS-PAGE |
| Source / Origin | Microbial source |
| pH Range / Optimal pH | Optimum pH 6.0-7.0; stable at pH 6.0-8.5 for 16 hours at 25 degrees C |
| Appearance | Yellow amorphous lyophilized powder |
| Alias | Lactate Oxidase |
| CAS Number | 9028-72-2 |
| Product Type | Lactate oxidase enzyme raw material |
| Function / Efficacy | Oxidize lactate and generate hydrogen peroxide for coupled lactate detection. |
| Abbreviation | LOX |
| EC Number | EC 1.1.3.2 |
| Specific Activity | >=45.0 U/mg dry powder |
| Isoelectric Point | 4.6 |
| Optimal Temperature | 50 degrees C |
| Thermal Stability | Stable below 50 degrees C at pH 7.0 for 30 minutes |
| Michaelis Constant | 7.5 x 10^-4 M for L-lactate |
| Detection Wavelength | 555 nm |
For research use only, not for clinical use.
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