>=45 U/mg Lactate Oxidase for 555 nm Lactate Detection
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>=45 U/mg Lactate Oxidase for 555 nm Lactate Detection

Cat.No: IEC-HMM-0224 Datasheet

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Product Name >=45 U/mg Lactate Oxidase for 555 nm Lactate Detection
Catalog No. IEC-HMM-0224
Description Alta DiagnoTech lactate oxidase is a yellow amorphous lyophilized enzyme powder for quantitative lactate detection.
Intended Use Enzyme raw material for lactate-content measurement.
Principle / Technology The generated quinoneimine dye is monitored spectrophotometrically at 555 nm.
Detection Method Coupled spectrophotometric assay at 555 nm
Performance Range / Specifications Dry-powder specific activity >=45.0 U/mg; purity >=90.0% by SDS-PAGE; pyruvate oxidase, choline oxidase, uricase and glucose oxidase each <=0.001%.
Storage Conditions Store at -25 to -15 degrees C
Shelf Life Maintains at least 90% activity for 12 months at -25 to -15 degrees C
Package Specifications 3 kU, 100 kU, 500 kU, 1,000 kU or custom packaging
Product Form Yellow amorphous lyophilized powder
Purity >=90.0% by SDS-PAGE
Activity / Unit Definition One unit is the amount of enzyme that generates 1 umol hydrogen peroxide per minute under the stated assay conditions.
Molecular Weight 42 kDa by SDS-PAGE
Source / Origin Microbial source
pH Range / Optimal pH Optimum pH 6.0-7.0; stable at pH 6.0-8.5 for 16 hours at 25 degrees C
Appearance Yellow amorphous lyophilized powder
Alias Lactate Oxidase
CAS Number 9028-72-2
Product Type Lactate oxidase enzyme raw material
Function / Efficacy Oxidize lactate and generate hydrogen peroxide for coupled lactate detection.
Abbreviation LOX
EC Number EC 1.1.3.2
Specific Activity >=45.0 U/mg dry powder
Isoelectric Point 4.6
Optimal Temperature 50 degrees C
Thermal Stability Stable below 50 degrees C at pH 7.0 for 30 minutes
Michaelis Constant 7.5 x 10^-4 M for L-lactate
Detection Wavelength 555 nm

For research use only, not for clinical use.

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