>=45 U/mg Hexokinase for Creatine Kinase and CK-MB Reagents

Name: >=45 U/mg Hexokinase for Creatine Kinase and CK-MB Reagents
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Cat.No: IEC-HMM-0213 Datasheet

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Product Name	>=45 U/mg Hexokinase for Creatine Kinase and CK-MB Reagents
Catalog No.	IEC-HMM-0213
Description	Alta DiagnoTech hexokinase is a white to pale-yellow amorphous lyophilized enzyme powder for NADH-generating creatine kinase and CK-MB reagent systems.
Intended Use	Enzyme raw material for research and bulk formulation of creatine kinase and creatine kinase-MB reagents.
Performance Range / Specifications	Specific activity >=45.0 U/mg dry powder; protein purity >=90%; NADH/NADPH oxidase <=0.01%; ATPase <=0.03%; glucose-6-phosphate dehydrogenase <=0.01%.
Storage Conditions	Store at -25 to -15 degrees C
Shelf Life	Maintains at least 90% activity for 12 months at -25 to -15 degrees C
Package Specifications	2 kU, 10 kU, 100 kU or 500 kU
Product Form	White to pale-yellow amorphous lyophilized powder
Purity	>=90% protein purity
Activity / Unit Definition	One unit is the amount of enzyme required to generate 1 umol NADH per minute under the stated conditions.
Molecular Weight	36 kDa by SDS-PAGE
Source / Origin	Microbial source
pH Range / Optimal pH	Optimum pH 8.0; stable at pH 6.5-9.0 for 16 hours at 25 degrees C
Appearance	White to pale-yellow amorphous lyophilized powder
Alias	Hexokinase
Product Type	Hexokinase enzyme raw material
Function / Efficacy	Phosphorylate glucose in coupled CK and CK-MB biochemical reagent systems.
Abbreviation	HK
EC Number	EC 2.7.1.1
Specific Activity	>=45.0 U/mg dry powder
Isoelectric Point	5.64
Inhibitors	SDS, Ag+, Fe3+, Hg2+ and Cu2+
Optimal Temperature	45 degrees C
Thermal Stability	Stable below 37 degrees C at pH 8.0 for 30 minutes
Michaelis Constant	3.2 x 10^-4 M for D-glucose; 8.3 x 10^-5 M for ATP
Stabilizer	BSA

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For research use only, not for clinical use.