>=45 U/mg Glucose-6-Phosphate Dehydrogenase for CK and CK-MB Reagents

Name: >=45 U/mg Glucose-6-Phosphate Dehydrogenase for CK and CK-MB Reagents
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Cat.No: IEC-HMM-0198 Datasheet

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Product Name	>=45 U/mg Glucose-6-Phosphate Dehydrogenase for CK and CK-MB Reagents
Catalog No.	IEC-HMM-0198
Description	Alta DiagnoTech glucose-6-phosphate dehydrogenase is a white amorphous lyophilized enzyme powder for NADH-generating creatine kinase and CK-MB reagent systems.
Intended Use	Enzyme raw material for research and bulk formulation of creatine kinase-MB and creatine kinase reagents.
Performance Range / Specifications	Specific activity >=45.0 U/mg dry powder; purity >=90% by SDS-PAGE; NADH/NADPH oxidase <=0.1%; hexokinase <=0.001%; glutathione reductase <=0.001%; phosphoglucose isomerase <=0.001%.
Storage Conditions	Store at -25 to -15 degrees C
Shelf Life	Maintains at least 90% activity for 12 months at -25 to -15 degrees C
Package Specifications	2 kU, 100 kU, 500 kU or 1,000 kU
Product Form	White amorphous lyophilized powder
Purity	>=90% by SDS-PAGE
Activity / Unit Definition	One unit is the amount of enzyme required to generate 1 umol NADH per minute under the stated conditions.
Molecular Weight	57 kDa by SDS-PAGE
Source / Origin	Microbial source
pH Range / Optimal pH	Optimum pH 8.0; stable at pH 5.5-10.0 for 16 hours at 25 degrees C
Appearance	White amorphous lyophilized powder
Alias	Glucose-6-Phosphate Dehydrogenase
Product Type	Glucose-6-phosphate dehydrogenase enzyme raw material
Function / Efficacy	Generate NADH in coupled CK and CK-MB biochemical reagent systems.
Abbreviation	G6PDH
EC Number	EC 1.1.1.49
Specific Activity	>=45.0 U/mg dry powder
Isoelectric Point	4.6
Inhibitors	SDS, Zn2+ and Fe3+
Optimal Temperature	45 degrees C
Thermal Stability	Stable below 40 degrees C at pH 7.5 for 30 minutes
Michaelis Constant	3.2 x 10^-4 M for NAD+; 2.6 x 10^-4 M for glucose-6-phosphate
Stabilizer	BSA

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For research use only, not for clinical use.