>=420 U/mg D-Lactate Dehydrogenase for ALT and AST Reagents

Name: >=420 U/mg D-Lactate Dehydrogenase for ALT and AST Reagents
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Cat.No: IEC-HMM-0181 Datasheet

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Product Name	>=420 U/mg D-Lactate Dehydrogenase for ALT and AST Reagents
Catalog No.	IEC-HMM-0181
Description	Alta DiagnoTech D-lactate dehydrogenase is a white amorphous lyophilized enzyme powder with low oxidase and dehydrogenase contaminants.
Intended Use	Enzyme raw material for aspartate aminotransferase and alanine aminotransferase reagent research and bulk formulation.
Principle / Technology	Activity is determined from NADH consumption at 340 nm.
Detection Method	Spectrophotometric NADH assay at 340 nm
Performance Range / Specifications	Dry-powder specific activity >=420.0 U/mg; purity >=90.0% by SDS-PAGE; NADH/NADPH oxidase <=0.01%; malate dehydrogenase <=0.005%; glutamate dehydrogenase <=0.003%; pyruvate kinase <=0.03%.
Storage Conditions	Store at -25 to -15 degrees C
Shelf Life	Maintains at least 90% activity for 12 months at -25 to -15 degrees C
Product Form	White amorphous lyophilized powder
Purity	>=90.0% by SDS-PAGE
Activity / Unit Definition	One unit is the amount of enzyme that oxidizes 1 umol NADH per minute under the stated assay conditions.
Molecular Weight	45 kDa by SDS-PAGE
Source / Origin	Microbial source
pH Range / Optimal pH	Optimum pH 6.5-7.0; stable at pH 5.5-10.0 for 16 hours at 25 degrees C
Appearance	White amorphous lyophilized powder
Alias	D-Lactate Dehydrogenase
Product Type	D-lactate dehydrogenase enzyme raw material
Function / Efficacy	Oxidize NADH in coupled transaminase reagent systems.
Abbreviation	D-LDH
EC Number	EC 1.1.1.28
Specific Activity	>=420.0 U/mg dry powder
Isoelectric Point	4.5
Inhibitors	Ag+ and Hg2+
Optimal Temperature	45 degrees C
Thermal Stability	Stable below 50 degrees C at pH 7.0 for 30 minutes
Michaelis Constant	2.2 x 10^-6 M for pyruvate
Stabilizer	BSA
Detection Wavelength	340 nm

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For research use only, not for clinical use.