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| Product Name | >=400 U/mg Creatinine Amidohydrolase for Enzymatic Creatinine Reagents |
| Catalog No. | IEC-HMM-0170 |
| Description | Alta DiagnoTech creatinine amidohydrolase is a white to pale-yellow amorphous lyophilized enzyme powder for hydrolysis of creatinine in enzymatic creatinine reagent systems. |
| Intended Use | Enzyme raw material for research and bulk formulation of enzymatic creatinine reagents. |
| Performance Range / Specifications | Dry-powder specific activity >=400.0 U/mg; purity >=90.0% by SDS-PAGE; catalase <=0.1%; protease, creatinase and ATPase each <=0.001%. |
| Storage Conditions | Store at -25 to -15 degrees C |
| Shelf Life | Maintains at least 90% activity for 12 months at -25 to -15 degrees C |
| Package Specifications | 10 kU, 100 kU, 1,000 kU or 10,000 kU |
| Product Form | White to pale-yellow amorphous lyophilized powder |
| Purity | >=90.0% by SDS-PAGE |
| Activity / Unit Definition | One unit is the amount of enzyme that generates 1 umol creatine per minute under the stated assay conditions. |
| Molecular Weight | 29 kDa by SDS-PAGE |
| Source / Origin | Microbial source |
| pH Range / Optimal pH | Optimum pH 7.0-8.0; stable at pH 5.5-10.0 for 16 hours at 25 degrees C |
| Appearance | White to pale-yellow amorphous lyophilized powder |
| Alias | Creatinine Amidohydrolase |
| CAS Number | 9025-13-2 |
| Product Type | Creatinine amidohydrolase enzyme raw material |
| Function / Efficacy | Hydrolyze creatinine to creatine in coupled enzymatic creatinine assays. |
| Abbreviation | CAH |
| EC Number | EC 3.5.2.10 |
| Specific Activity | >=400.0 U/mg dry powder |
| Isoelectric Point | 5.3 |
| Inhibitors | Hg2+, Cu2+ and Fe3+ |
| Optimal Temperature | 65 degrees C |
| Thermal Stability | Stable below 65 degrees C at pH 8.0 for 30 minutes |
| Michaelis Constant | 5.0 x 10^2 M for creatinine; 8.0 x 10^2 M for creatine |
| Stabilizer | Sugars and BSA |
For research use only, not for clinical use.
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