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| Product Name | >=40 U/mg Beta-Galactosidase for 405 nm ONPG Activity Assays |
| Catalog No. | IEC-HMM-0154 |
| Description | Alta DiagnoTech beta-galactosidase is a white amorphous enzyme powder characterized for ONPG-based biochemical reagent development. |
| Intended Use | Enzyme raw material for biochemical assay development, including the listed sodium-assay-kit application. |
| Principle / Technology | Beta-galactosidase hydrolyzes o-nitrophenyl beta-D-galactoside, and product formation is monitored at 405 nm. |
| Detection Method | Kinetic ONPG absorbance assay at 405 nm |
| Performance Range / Specifications | Specific activity is at least 40 U/mg; optimal assay range is 100-1,000 U/L. |
| Reaction Conditions / Protocol | Preincubate 0.520 mL assay reagent with 0.020 mL diluted enzyme for 1 minute at 37 degrees C, add 0.060 mL of 22.7 mM ONPG, react for 4 minutes at 37 degrees C and calculate the A405 change from minute 3 to minute 4. Total reaction volume is 0.6 mL. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | At least 1 year at -20 degrees C |
| Product Form | White amorphous powder |
| Activity / Unit Definition | One unit hydrolyzes 1.0 umol ONPG substrate per minute under the stated assay conditions. |
| Molecular Weight | 116.5 kDa |
| pH Range / Optimal pH | Stable at pH 5.0-9.5; optimum pH 7.5 |
| Recommended Buffer System | Assay reagent combines 100 mM sodium phosphate at pH 7.3, beta-mercaptoethanol and 30 mM MgCl2 in an 8:1:1 ratio. Enzyme diluent is 50 mM phosphate at pH 7.3 with 1.0 mM MgCl2 and 0.1% BSA. |
| Appearance | White amorphous powder |
| Alias | Beta-Galactosidase |
| Product Type | Beta-galactosidase enzyme raw material |
| Function / Efficacy | Hydrolyze beta-D-galactosides for coupled biochemical assays. |
| Abbreviation | GAH |
| Customization | Specifications can be customized |
| Specific Activity | >=40 U/mg |
| Isoelectric Point | 5.28 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | 55 degrees C |
| Thermal Stability | Stable below 50 degrees C in the presence of 1 mM MgCl2 |
| Working Solution | Dilute to 100-1,000 U/L with enzyme diluent and hold at room temperature for 1 hour before measurement. |
| Michaelis Constant | 4.33 x 10^-4 M for 2-nitrophenyl beta-D-galactoside |
| Detection Wavelength | 405 nm |
| Linear Range | 100-1,000 U/L |
For research use only, not for clinical use.
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