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| Product Name | ≥4 U/mg Fructosyl-Peptide Oxidase for Glycated-Hemoglobin Reagents |
| Catalog No. | IEC-HMM-0191 |
| Description | Alta DiagnoTech fructosyl-peptide oxidase is a high-purity yellow lyophilized enzyme for glycated-hemoglobin biochemical reagent formulation. |
| Intended Use | For oxidation of glycated amino acids and for research, development and large-scale formulation of enzymatic glycated-hemoglobin reagents. |
| Performance Range / Specifications | Catalase: ≤0.01%; ATPase: ≤0.005%; glucose oxidase: ≤0.03%; cholesterol oxidase: ≤0.003%. |
| Storage Conditions | Store without agitation at -25°C to -15°C. |
| Shelf Life | Retains at least 90% activity for 12 months under the stated storage conditions. |
| Package Specifications | Available in 1 kU, 10 kU and 50 kU packs. |
| Product Form | Lyophilized enzyme powder |
| Purity | ≥90% by SDS-PAGE |
| Activity / Unit Definition | One unit is the amount of enzyme that produces 1 µmol of hydrogen peroxide per minute under the specified reaction conditions. |
| Molecular Weight | 60 kDa by SDS-PAGE |
| Source / Origin | Microbial |
| pH Range / Optimal pH | Optimum pH: 6.5-7.5; stable at pH 6.5-9.5 after 16 hours at 25°C. |
| Appearance | Yellow amorphous lyophilized powder |
| Alias | Fructosyl-peptide oxidase |
| Product Type | Diagnostic raw-material enzyme |
| Function / Efficacy | Oxidizes fructosyl-peptide substrates and generates hydrogen peroxide for coupled detection. |
| Abbreviation | FPOX |
| EC Number | EC 1.5.3 |
| Specific Activity | ≥4 U/mg enzyme powder |
| Isoelectric Point | 6.4 |
| Inhibitors | Hg2+ and Pb2+ |
| Optimal Temperature | 37°C |
| Thermal Stability | Stable below 40°C at pH 8.0 for 30 minutes. |
| Michaelis Constant | 4.0 × 10^-3 M for fructosyl-Val-His. |
| Stabilizer | Glycerol and trehalose |
For research use only, not for clinical use.
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