≥4 U/mg Fructosyl-Peptide Oxidase for Glycated-Hemoglobin Reagents
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≥4 U/mg Fructosyl-Peptide Oxidase for Glycated-Hemoglobin Reagents

Cat.No: IEC-HMM-0191 Datasheet

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Product Name ≥4 U/mg Fructosyl-Peptide Oxidase for Glycated-Hemoglobin Reagents
Catalog No. IEC-HMM-0191
Description Alta DiagnoTech fructosyl-peptide oxidase is a high-purity yellow lyophilized enzyme for glycated-hemoglobin biochemical reagent formulation.
Intended Use For oxidation of glycated amino acids and for research, development and large-scale formulation of enzymatic glycated-hemoglobin reagents.
Performance Range / Specifications Catalase: ≤0.01%; ATPase: ≤0.005%; glucose oxidase: ≤0.03%; cholesterol oxidase: ≤0.003%.
Storage Conditions Store without agitation at -25°C to -15°C.
Shelf Life Retains at least 90% activity for 12 months under the stated storage conditions.
Package Specifications Available in 1 kU, 10 kU and 50 kU packs.
Product Form Lyophilized enzyme powder
Purity ≥90% by SDS-PAGE
Activity / Unit Definition One unit is the amount of enzyme that produces 1 µmol of hydrogen peroxide per minute under the specified reaction conditions.
Molecular Weight 60 kDa by SDS-PAGE
Source / Origin Microbial
pH Range / Optimal pH Optimum pH: 6.5-7.5; stable at pH 6.5-9.5 after 16 hours at 25°C.
Appearance Yellow amorphous lyophilized powder
Alias Fructosyl-peptide oxidase
Product Type Diagnostic raw-material enzyme
Function / Efficacy Oxidizes fructosyl-peptide substrates and generates hydrogen peroxide for coupled detection.
Abbreviation FPOX
EC Number EC 1.5.3
Specific Activity ≥4 U/mg enzyme powder
Isoelectric Point 6.4
Inhibitors Hg2+ and Pb2+
Optimal Temperature 37°C
Thermal Stability Stable below 40°C at pH 8.0 for 30 minutes.
Michaelis Constant 4.0 × 10^-3 M for fructosyl-Val-His.
Stabilizer Glycerol and trehalose

For research use only, not for clinical use.

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