3alpha-Hydroxysteroid Dehydrogenase 3alpha-HSD Enzyme for Total Bile Acid Assay >=20 U/mg 30 kDa CAS 9028-56-2

Name: 3alpha-Hydroxysteroid Dehydrogenase 3alpha-HSD Enzyme for Total Bile Acid Assay >=20 U/mg 30 kDa CAS 9028-56-2
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Cat.No: IEC-HMM-0283 Datasheet

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Product Name	3alpha-Hydroxysteroid Dehydrogenase 3alpha-HSD Enzyme for Total Bile Acid Assay >=20 U/mg 30 kDa CAS 9028-56-2
Catalog No.	IEC-HMM-0283
Description	3alpha-Hydroxysteroid dehydrogenase from Alta DiagnoTech catalyzes the oxidation of 3alpha-hydroxysteroids. It is the key enzyme in total bile acid (TBA) assay kits used for liver function evaluation.
Intended Use	Core enzyme for total bile acid diagnostic reagents and steroid metabolism research.
Principle / Technology	Oxidizes 3alpha-hydroxyl groups on bile acids with NAD or NADP cofactors; the NADH produced is proportional to bile acid concentration.
Detection Method	NADH absorbance at 340 nm.
Sample Type	Serum, plasma.
Performance Range / Specifications	Specific activity >=20 U/mg. Molecular weight approximately 30 kDa by SDS-PAGE. Isoelectric point 5.0.
Components / Formulation	3alpha-hydroxysteroid dehydrogenase lyophilized powder.
Storage Conditions	-20°C.
Shelf Life	Stable for at least 12 months at -20°C.
Package Specifications	KU packaging units.
Product Form	White to pale yellow amorphous powder.
Purity	99%
Activity / Unit Definition	>=20 U/mg
Molecular Weight	Approximately 30 kDa (SDS-PAGE)
Source / Origin	Microbial fermentation.
pH Range / Optimal pH	Optimal pH 10; stable between pH 6.0-10.0.
Shipping Conditions	Cold chain shipment.
Expiration Date / Stability	-20°C stable for one year.
Appearance	White to pale yellow amorphous powder.
CAS Number	9028-56-2
Isoelectric Point	5.0
Inhibitors	Not inhibited by sodium azide.
Optimal Temperature	55°C.
Thermal Stability	Stable below 50°C.
Handling Precaution	Avoid repeated freeze-thaw; keep dry.

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For research use only, not for clinical use.