- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | >=3,000 kU/L Recombinant Cystathionine Beta-Lyase for Homocysteine Assays |
| Catalog No. | IEC-HMM-0175 |
| Description | Alta DiagnoTech cystathionine beta-lyase is a recombinant enzyme supplied as a bright-yellow clear liquid or yellow lyophilized powder for cystathionine-pathway homocysteine assays. |
| Intended Use | Key enzyme ingredient for L-homocysteine IVD assays based on the L-cystathionine pathway, used with cystathionine beta-synthase and D-lactate dehydrogenase. |
| Principle / Technology | CBL converts L-cystathionine to L-homocysteine, pyruvate and ammonia. Coupled D-lactate dehydrogenase converts pyruvate and NADH to lactate and NAD+, enabling kinetic monitoring. |
| Detection Method | NADH rate assay at 340 nm |
| Performance Range / Specifications | Liquid activity concentration >=3,000 kU/L; protein specific activity about 350 U/mg; lyophilized powder specific activity >=35 U/mg; SDS-PAGE purity >80%. |
| Reaction Conditions / Protocol | Measure the reverse reaction with L-cystathionine at pH 9.0 and 37 degrees C, using the NADH absorbance-change rate at 340 nm. The stated NADH millimolar extinction coefficient is 6.22 cm2/umol. |
| Components / Formulation | Liquid: 50 mM potassium phosphate at pH 7.0 with 50% glycerol, stabilizer and 0.1% sodium azide. A yellow lyophilized-powder format is also available. |
| Storage Conditions | Store frozen at -20 degrees C |
| Shelf Life | At least 18 months at -20 degrees C |
| Product Form | Bright-yellow clear liquid or yellow lyophilized powder |
| Purity | >80% by SDS-PAGE |
| Activity / Unit Definition | One unit converts 1 umol L-cystathionine to 1 umol L-homocysteine per minute at pH 9.0 and 37 degrees C, with coupled consumption of 1 umol NADH. |
| Molecular Weight | Approximately 40 kDa by SDS-PAGE |
| Source / Origin | Microbial gene, recombinantly expressed in E. coli |
| pH Range / Optimal pH | Stable at pH 6.5-8.0 for 24 hours at 25 degrees C; optimum pH 8.5-10.5 |
| Alias | Cystathionine beta-lyase |
| Safety | Contains sodium azide preservative; use appropriate contact protection. |
| Product Type | Cystathionine beta-lyase enzyme raw material |
| Function / Efficacy | Release L-homocysteine and pyruvate from L-cystathionine for coupled homocysteine measurement. |
| Abbreviation | CBL |
| Customization | Specifications can be customized |
| Specific Activity | About 350 U/mg protein; >=35 U/mg lyophilized powder |
| Isoelectric Point | 6.4 |
| Inhibitors | Activity is not inhibited by 0.1% sodium azide or phosphate |
| Optimal Temperature | 45 degrees C |
| Thermal Stability | Stable at <=55 degrees C with stabilizer at pH 7.5 for 30 minutes; liquid and powder remain stable in accelerated studies at 42 degrees C for 3 days or 37 degrees C for 14 days |
| Activator | 5'-pyridoxal phosphate cofactor |
| Stabilizer | Glycerol, Triton X-100 and BSA |
| Detection Wavelength | 340 nm |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
