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| Product Name | ≥300 U/mg β-Hydroxybutyrate Dehydrogenase for Ketone Assays |
| Catalog No. | IEC-HMM-0265 |
| Description | Alta DiagnoTech microbial β-hydroxybutyrate dehydrogenase is a white lyophilized enzyme powder for development and scale-up of β-hydroxybutyrate reagents. |
| Intended Use | For development and large-scale preparation of β-hydroxybutyrate reagents. |
| Storage Conditions | Store undisturbed at -25°C to -15°C. |
| Package Specifications | 5 kU, 50 kU and 100 kU |
| Product Form | Lyophilized enzyme powder |
| Purity | ≥90% protein purity |
| Activity / Unit Definition | One unit is the amount of enzyme that generates 1 µmol NADH per minute under the stated reaction conditions. |
| Molecular Weight | 27 kDa by SDS-PAGE |
| Source / Origin | Microbial |
| pH Range / Optimal pH | Optimal pH 8.5; stable at pH 5.0-10.0 for 16 hours at 25°C |
| Expiration Date / Stability | Retains more than 90% activity for 12 months at -25°C to -15°C. |
| Appearance | White amorphous lyophilized powder |
| Alias | β-Hydroxybutyrate Dehydrogenase |
| CAS Number | 9028-38-0 |
| Product Type | β-Hydroxybutyrate dehydrogenase raw material |
| Function / Efficacy | Catalyzes the NAD+-dependent reaction used in β-hydroxybutyrate assays. |
| Abbreviation | β-HBDH |
| EC Number | 1.1.1.30 |
| Specific Activity | ≥300 U/mg enzyme powder |
| Isoelectric Point | 6.1 |
| Inhibitors | Hg2+, Ag+ and SDS |
| Optimal Temperature | 60°C |
| Thermal Stability | Stable below 50°C for 30 minutes at pH 8.5 |
| Michaelis Constant | 1.46 × 10^-2 M for D-3-hydroxybutyrate; 9.5 × 10^-5 M for NAD+ |
| Interference | Malate dehydrogenase ≤0.002%; lactate dehydrogenase ≤0.002%; NADH oxidase ≤0.002%. |
For research use only, not for clinical use.
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