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| Product Name | ≥30 U/mg Recombinant Microbial Transglutaminase for Antibody Conjugation |
| Catalog No. | IEC-HMM-0249 |
| Description | Alta DiagnoTech recombinant microbial transglutaminase catalyzes acyl transfer from glutamine side chains to primary amines, enabling intra- and intermolecular protein crosslinking without calcium. |
| Intended Use | For research in recombinant-antibody conjugation, protein modification, protein labeling, crosslinking and immobilization. |
| Principle / Technology | The enzyme recognizes LLQG and transfers the γ-carboxamide acyl group of glutamine residues to primary amines, including lysine side chains, without requiring Ca2+. |
| Storage Conditions | Store at -80°C to -20°C and minimize repeated freeze-thaw cycles. |
| Shelf Life | 1 year |
| Package Specifications | 1 mg, 5 mg and 50 mg |
| Product Form | Recombinant enzyme solution |
| Purity | ≥95% by SEC-HPLC |
| Source / Origin | Sequence derived from Streptoverticillium mobaraense and expressed recombinantly in Escherichia coli |
| pH Range / Optimal pH | Optimal pH 7.0-7.5 |
| Recommended Buffer System | PB or HEPES buffer; avoid buffers containing primary amines |
| Alias | Recombinant Microbial Transglutaminase |
| Safety | For research use only; not for medical, food or industrial-scale production use. |
| Product Type | Calcium-independent microbial transglutaminase |
| Function / Efficacy | Catalyzes covalent protein and peptide crosslinking for conjugation and labeling research. |
| Abbreviation | mTGase |
| Specific Activity | ≥30 U/mg |
| Optimal Temperature | 45°C to 50°C |
| Storage Buffer | 20 mM HEPES, 300 mM NaCl, pH 7.0 |
For research use only, not for clinical use.
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