- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | ≥30 U/mg Proteinase K with No Detected DNase or RNase Activity |
| Catalog No. | IEC-HMM-0241 |
| Description | Alta DiagnoTech proteinase K is a broad-specificity serine protease that preferentially cleaves ester and peptide bonds adjacent to hydrophobic, sulfur-containing or aromatic amino-acid residues. |
| Intended Use | For nucleic acid extraction kits, genetic diagnostic reagents, removal of protein impurities, chromosome DNA preparation for pulsed-field electrophoresis, western blot workflows and enzymatic glycated-albumin reagent development. |
| Performance Range / Specifications | DNase activity: not detected; RNase activity: not detected; nicking activity: not detected. |
| Storage Conditions | Store the dry powder at 8°C or below. |
| Shelf Life | 36 months in the dry state at 8°C or below. |
| Package Specifications | Available in 100 mg, 1 g, 10 g and 100 g packs; custom packing is supported. |
| Product Form | Lyophilized enzyme powder |
| Activity / Unit Definition | One unit is the amount of enzyme that hydrolyzes casein to produce 1 µmol of L-tyrosine per minute under the specified conditions. |
| Molecular Weight | 29 kDa by SDS-PAGE |
| Source / Origin | Tritirachium album |
| pH Range / Optimal pH | High activity from pH 7.0 to 12.0; stable at pH 4.5-12.5 after 16 hours at 25°C. |
| Appearance | White to off-white amorphous lyophilized powder |
| Alias | Proteinase K |
| Safety | Wear protective gloves and eye protection. The material may cause an allergic skin reaction; inhalation may trigger allergy or asthma symptoms or breathing difficulty. Use with good ventilation. |
| Product Type | Broad-specificity serine protease |
| Function / Efficacy | Degrades proteins into shorter peptides across a broad range of reaction conditions. |
| Abbreviation | PRK |
| EC Number | EC 3.4.21.64 |
| Specific Activity | ≥30 U/mg |
| Isoelectric Point | 7.81 |
| Inhibitors | Diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride |
| Optimal Temperature | 65°C |
| Thermal Stability | Stable below 50°C at pH 8.0 for 30 minutes; retains more than 90% activity for one year at 25°C. |
| Physical Properties | Contains the serine-protease catalytic triad Asp39-His69-Ser224 and two calcium-binding sites near the active center. |
| Activator | SDS and urea |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
