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| Product Name | >=30 U/mg Glycerol Kinase for 505 nm Triglyceride Assays |
| Catalog No. | IEC-HMM-0202 |
| Description | Alta DiagnoTech glycerol kinase is a pale-yellow amorphous enzyme powder for triglyceride biochemical reagent development. |
| Intended Use | Enzyme raw material for biochemical triglyceride assay kits. |
| Detection Method | Glycerol-phosphate-oxidase/peroxidase-coupled absorbance assay at 505 nm |
| Performance Range / Specifications | Specific activity >=30 U/mg; assay linear range 100-400 U/L. |
| Reaction Conditions / Protocol | Use HEPES reagent at pH 7.9 containing glycerol, phenol, MgCl2 and peroxidase, plus a second HEPES reagent containing ATP, 4-aminoantipyrine, BSA and glycerol-phosphate oxidase. Combine 1.0 mL reagent 1 and 0.2 mL reagent 2, preincubate for 180 seconds at 37 degrees C, add 0.012 mL sample and determine the one-minute rate after 2 minutes at 505 nm. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | At least 1 year at -20 degrees C |
| Product Form | Pale-yellow amorphous powder |
| Activity / Unit Definition | One unit produces 1 umol hydrogen peroxide, equivalent to 0.5 umol quinone dye, per minute under the stated conditions. |
| Molecular Weight | 55 kDa |
| pH Range / Optimal pH | Stable at pH 6.0-10.0; optimum pH 8.0 |
| Appearance | Pale-yellow amorphous powder |
| Alias | Glycerol kinase |
| Product Type | Glycerol kinase enzyme raw material |
| Function / Efficacy | Phosphorylate glycerol for coupled triglyceride measurement. |
| Abbreviation | GK |
| Customization | Specifications can be customized |
| Specific Activity | >=30 U/mg |
| Isoelectric Point | 4.3 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | 50 degrees C |
| Thermal Stability | Stable to 60 degrees C |
| Michaelis Constant | 1.3 x 10^-5 M for ATP; 5.2 x 10^-5 M for glycerol |
| Detection Wavelength | 505 nm |
| Linear Range | 100-400 U/L |
For research use only, not for clinical use.
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