>=30 U/mg Glycerol Kinase for 505 nm Triglyceride Assays

Name: >=30 U/mg Glycerol Kinase for 505 nm Triglyceride Assays
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Cat.No: IEC-HMM-0202 Datasheet

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Product Name	>=30 U/mg Glycerol Kinase for 505 nm Triglyceride Assays
Catalog No.	IEC-HMM-0202
Description	Alta DiagnoTech glycerol kinase is a pale-yellow amorphous enzyme powder for triglyceride biochemical reagent development.
Intended Use	Enzyme raw material for biochemical triglyceride assay kits.
Detection Method	Glycerol-phosphate-oxidase/peroxidase-coupled absorbance assay at 505 nm
Performance Range / Specifications	Specific activity >=30 U/mg; assay linear range 100-400 U/L.
Reaction Conditions / Protocol	Use HEPES reagent at pH 7.9 containing glycerol, phenol, MgCl2 and peroxidase, plus a second HEPES reagent containing ATP, 4-aminoantipyrine, BSA and glycerol-phosphate oxidase. Combine 1.0 mL reagent 1 and 0.2 mL reagent 2, preincubate for 180 seconds at 37 degrees C, add 0.012 mL sample and determine the one-minute rate after 2 minutes at 505 nm.
Storage Conditions	Store at -20 degrees C
Shelf Life	At least 1 year at -20 degrees C
Product Form	Pale-yellow amorphous powder
Activity / Unit Definition	One unit produces 1 umol hydrogen peroxide, equivalent to 0.5 umol quinone dye, per minute under the stated conditions.
Molecular Weight	55 kDa
pH Range / Optimal pH	Stable at pH 6.0-10.0; optimum pH 8.0
Appearance	Pale-yellow amorphous powder
Alias	Glycerol kinase
Product Type	Glycerol kinase enzyme raw material
Function / Efficacy	Phosphorylate glycerol for coupled triglyceride measurement.
Abbreviation	GK
Customization	Specifications can be customized
Specific Activity	>=30 U/mg
Isoelectric Point	4.3
Inhibitors	Not inhibited by sodium azide
Optimal Temperature	50 degrees C
Thermal Stability	Stable to 60 degrees C
Michaelis Constant	1.3 x 10^-5 M for ATP; 5.2 x 10^-5 M for glycerol
Detection Wavelength	505 nm
Linear Range	100-400 U/L

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For research use only, not for clinical use.