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| Product Name | >=30 U/mg Cystathionine Beta-Lyase for Homocysteine Reagents |
| Catalog No. | IEC-HMM-0176 |
| Description | Alta DiagnoTech cystathionine beta-lyase is a yellow amorphous lyophilized enzyme powder for NADH-coupled homocysteine reagent systems. |
| Intended Use | Enzyme raw material for research and bulk formulation of homocysteine reagents. |
| Performance Range / Specifications | Specific activity >=30.0 U/mg dry powder; purity >=90.0% by SDS-PAGE; glucose-6-phosphate dehydrogenase <=0.01%; lactate dehydrogenase <=0.01%. |
| Storage Conditions | Store at -25 to -15 degrees C |
| Shelf Life | Maintains at least 90% activity for 12 months at -25 to -15 degrees C |
| Package Specifications | 5 kU, 50 kU or 500 kU |
| Product Form | Yellow amorphous lyophilized powder |
| Purity | >=90.0% by SDS-PAGE |
| Activity / Unit Definition | One unit is the amount of enzyme required to consume 1 umol NADH per minute under the stated activity-assay conditions. |
| Molecular Weight | 54 kDa by SDS-PAGE |
| Source / Origin | Microbial source |
| pH Range / Optimal pH | Optimum pH 8.0; stable at pH 6.0-10.0 for 16 hours at 25 degrees C |
| Appearance | Yellow amorphous lyophilized powder |
| Alias | Cystathionine Beta-Lyase |
| Product Type | Cystathionine beta-lyase enzyme raw material |
| Function / Efficacy | Provide cystathionine beta-lyase activity in coupled homocysteine reagent systems. |
| Abbreviation | CBL |
| EC Number | EC 4.4.1.8 |
| Specific Activity | >=30.0 U/mg dry powder |
| Isoelectric Point | 6.0 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | 50 degrees C |
| Thermal Stability | Stable below 45 degrees C at pH 8.0 for 30 minutes |
| Michaelis Constant | 1.6 x 10^-3 M for L-cystathionine |
| Stabilizer | Triton X-100 and glycerol |
For research use only, not for clinical use.
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