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| Product Name | 30-Second Activated Dual-Blocked Hot-Start Taq for Fast Multiplex qPCR |
| Catalog No. | IEC-HMM-0187 |
| Description | Alta DiagnoTech dual-blocked hot-start Taq polymerase uses antibody control of both 5'-to-3' polymerase and 5'-to-3' exonuclease activity at room temperature. |
| Intended Use | For fast singleplex or multiplex quantitative PCR using DNA templates, including systems in which primers and probes are premixed. |
| Principle / Technology | Antibody modification suppresses polymerase and exonuclease activities at room temperature, reducing nonspecific amplification, primer dimers and premature primer or probe degradation. Heating at 95°C for 30 seconds releases full enzyme activity. |
| Performance Range / Specifications | Complete activation at 95°C for 30 seconds. |
| Reaction Conditions / Protocol | Activate at 95°C for 30 seconds before amplification. |
| Components / Formulation | Each format includes antibody-modified hot-start Taq DNA polymerase and a 5× Taq buffer. The 1 kU, 10 kU and 100 kU formats contain 50 µL, 500 µL and 5 mL enzyme, respectively, with matching buffer volumes of 2 × 1 mL, 2 × 10 mL and 2 × 100 mL. |
| Storage Conditions | Store at -25°C to -15°C. |
| Package Specifications | Available in 1 kU, 10 kU and 100 kU formats. |
| Key Features | Dual activity blockade, rapid activation, primer/probe premix support, high specificity and high amplification sensitivity. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | Compatible with fast PCR programs and existing PCR cycling protocols. |
| Alias | Dual-blocked hot-start Taq DNA polymerase |
| Product Type | Antibody-modified hot-start Taq DNA polymerase |
| Function / Efficacy | Supports rapid, sensitive and specific DNA amplification while protecting premixed primers and probes before thermal activation. |
For research use only, not for clinical use.
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