>=3 U/mg Fructosyl Peptide Oxidase for Enzymatic HbA1c Assays

Name: >=3 U/mg Fructosyl Peptide Oxidase for Enzymatic HbA1c Assays
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Cat.No: IEC-HMM-0190 Datasheet

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Product Name	>=3 U/mg Fructosyl Peptide Oxidase for Enzymatic HbA1c Assays
Catalog No.	IEC-HMM-0190
Description	Alta DiagnoTech fructosyl peptide oxidase is a yellow amorphous enzyme powder for enzymatic glycated-hemoglobin reagent development.
Intended Use	Enzyme raw material for enzymatic HbA1c assay kits.
Detection Method	Peroxidase-coupled kinetic absorbance assay at 555 nm
Performance Range / Specifications	Specific activity >=3 U/mg; assay range 100-600 U/L.
Reaction Conditions / Protocol	Use 0.1 M potassium phosphate at pH 8.0, TOOS, peroxidase/4-aminoantipyrine and fructosyl valine. Preincubate 540 uL peroxidase/4-AA reagent, 20 uL TOOS and 20 uL sample for 2 minutes at 37 degrees C, add 20 uL fructosyl-valine solution, react for 5 minutes and determine the linear rate at 555 nm with a buffer blank.
Storage Conditions	Store at -20 degrees C
Shelf Life	At least 1 year at -20 degrees C
Product Form	Yellow amorphous powder
Activity / Unit Definition	One unit produces 1 umol hydrogen peroxide per minute under the stated reaction conditions.
Molecular Weight	52 kDa by SDS-PAGE
pH Range / Optimal pH	Stable at pH 6.0-8.0; optimum pH 7.5
Appearance	Yellow amorphous powder
Alias	Fructosyl peptide oxidase
Product Type	Fructosyl peptide oxidase enzyme raw material
Function / Efficacy	Oxidize fructosyl peptides and generate hydrogen peroxide for HbA1c-related enzymatic detection.
Abbreviation	FAOD-I
Customization	Specifications can be customized
Specific Activity	>=3 U/mg
Inhibitors	Not affected by sodium azide
Optimal Temperature	30-40 degrees C
Thermal Stability	Stable below 40 degrees C
Stabilizer	EDTA
Detection Wavelength	555 nm
Linear Range	100-600 U/L

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For research use only, not for clinical use.