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| Product Name | >=25 U/mg 3alpha-Hydroxysteroid Dehydrogenase for Total Bile Acid Assays |
| Catalog No. | IEC-HMM-0136 |
| Description | Alta DiagnoTech 3alpha-hydroxysteroid dehydrogenase is an off-white amorphous enzyme powder for total bile acid reagent development. |
| Intended Use | Enzyme raw material for total bile acid biochemical assay kits. |
| Detection Method | Kinetic NADH absorbance assay at 340 nm |
| Performance Range / Specifications | Specific activity >=25 U/mg; assay linear range 40-200 U/L. |
| Reaction Conditions / Protocol | Use sodium pyrophosphate buffer at pH 8.90, NAD and androsterone in methanol. Combine 2.6 mL buffer, 0.2 mL NAD solution and 0.1 mL sample, equilibrate at 25 degrees C, add 0.1 mL androsterone substrate and measure the absorbance-change rate from 30 to 70 seconds at 340 nm. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | 1 year at -20 degrees C |
| Product Form | Off-white amorphous powder |
| Activity / Unit Definition | One unit converts 1 umol androsterone per minute at 25 degrees C and pH 8.9. |
| Molecular Weight | Approximately 26 kDa by SDS-PAGE |
| pH Range / Optimal pH | Stable at pH 6.0-10.0; optimum pH 10.0 |
| Appearance | Off-white amorphous powder |
| Alias | 3alpha-Hydroxysteroid dehydrogenase |
| Product Type | 3alpha-hydroxysteroid dehydrogenase enzyme raw material |
| Function / Efficacy | Catalyze hydroxysteroid oxidation for total bile acid assays. |
| Abbreviation | 3-HSD |
| Customization | Specifications can be customized |
| Specific Activity | >=25 U/mg |
| Isoelectric Point | 5.3 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | 55 degrees C |
| Thermal Stability | Stable to 50 degrees C |
| Detection Wavelength | 340 nm |
| Linear Range | 40-200 U/L |
For research use only, not for clinical use.
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