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| Product Name | >=2,000 U/mg High-Activity Alkaline Phosphatase for Antibody Labeling |
| Catalog No. | IEC-HMM-0145 |
| Description | Alta DiagnoTech high-activity alkaline phosphatase is a colorless to light-red solution suitable for antibody labeling, chemiluminescence reagent development and ALP controls. |
| Intended Use | ALP assay calibrator or quality-control material and enzyme label for antibodies and general chemiluminescence reagents. |
| Principle / Technology | ALP hydrolyzes colorless disodium p-nitrophenyl phosphate to pale-yellow p-nitrophenol, whose formation is monitored photometrically. |
| Detection Method | SFBC kinetic absorbance assay at 405 or 410 nm |
| Performance Range / Specifications | Specific activity is at least 2,000 U/mg protein, purity is at least 95% by SDS-PAGE and the stated assay range is 0-600 U/L. |
| Reaction Conditions / Protocol | Use R1:sample:R2 at 1,000:20:200, incubate at 37 degrees C for 60 seconds and measure the A405 or A410 increase over the following 120 seconds; stated factor 3,298. |
| Storage Conditions | Store at 2-8 degrees C and avoid freeze-thaw cycles |
| Shelf Life | At least 2 years at 2-8 degrees C |
| Product Form | Colorless to light-red solution; lyophilized form is stable with PBS and BSA |
| Purity | >=95% by SDS-PAGE |
| Molecular Weight | Approximately 56 kDa |
| pH Range / Optimal pH | Stable at pH 6.5-11.5 for 24 hours at 25 degrees C; optimum pH 11.0-11.2 |
| Recommended Buffer System | 50 mM potassium phosphate, 1 mM MgCl2, 0.1 mM CoCl2 and 0.1% NaN3 at pH 7.5 |
| Appearance | Colorless to light-red solution |
| Alias | Alkaline Phosphatase, High Activity |
| Product Type | High-activity alkaline phosphatase raw material |
| Function / Efficacy | Provide high ALP activity for antibody conjugation, chemiluminescence and control materials. |
| Abbreviation | ALP |
| Customization | Specifications can be customized |
| Specific Activity | >=2,000 U/mg protein |
| Isoelectric Point | Approximately 4.62 |
| Inhibitors | EDTA and EGTA |
| Optimal Temperature | Activity increases from 20 to 60 degrees C; the value at 37 degrees C is 3.3 times the value at 25 degrees C |
| Thermal Stability | Stable below 45 degrees C; retains more than 50% activity after 30 minutes at 60 degrees C; inactive above 70 degrees C |
| Activator | 0.1 mM CoCl2 |
| Detection Wavelength | 405 or 410 nm |
| Linear Range | 0-600 U/L |
For research use only, not for clinical use.
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