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| Product Name | 200 U/µL Room-Temperature-Blocked Reverse Transcriptase for One-Step RT-qPCR |
| Catalog No. | IEC-HMM-0234 |
| Description | Alta DiagnoTech hot-start reverse transcriptase is a reversibly modified M-MLV-derived enzyme designed for room-temperature reaction setup and high-temperature cDNA synthesis. |
| Intended Use | For one-step RT-qPCR and fully premixed RNA amplification reagents. |
| Principle / Technology | Reversible modification suppresses enzyme activity at room temperature. Activity is released at 55°C without a separate activation step. |
| Storage Conditions | Store at -25°C to -15°C. |
| Package Specifications | Available in 10 kU, 100 kU and 2,000 kU formats. |
| Product Form | Liquid reverse transcriptase |
| Concentration | 200 U/µL |
| Source / Origin | Moloney murine leukemia virus |
| Shipping Conditions | Ship at 0°C or below. |
| Application Notes / Precautions | Use RNase-free tubes and tips. cDNA may be stored at -20°C or -80°C or used immediately; when added to PCR or qPCR, keep cDNA volume at no more than one tenth of the PCR reaction volume. |
| Alias | M-MLV reverse transcriptase |
| Product Type | Hot-start reverse transcriptase |
| Function / Efficacy | Provides stable, temperature-tolerant reverse transcription while limiting nonspecific activity during room-temperature setup. |
For research use only, not for clinical use.
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