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| Product Name | >=20 U/mg NAD-Dependent Glutamate Dehydrogenase for Ammonia Assays |
| Catalog No. | IEC-HMM-0199 |
| Description | Alta DiagnoTech NAD-dependent glutamate dehydrogenase is an off-white amorphous enzyme powder for urea nitrogen, blood ammonia and potassium reagent development. |
| Intended Use | Enzyme raw material for biochemical assays of urea nitrogen, blood ammonia and potassium. |
| Detection Method | Kinetic NADH absorbance assay at 340 nm |
| Performance Range / Specifications | Specific activity >=20 U/mg; assay linear range 100-1,000 U/L with 100-500 U/L recommended. |
| Reaction Conditions / Protocol | Use Tris reagent at pH 8.30 containing ammonium chloride and alpha-ketoglutarate, together with a CAPS/NADH reagent at pH 9.6. Add 0.9 mL reagent 1 and 0.18 mL reagent 2, preincubate for 60 seconds at 37 degrees C, add 0.015 mL sample after 4 minutes and measure the one-minute rate at 340 nm. Complete measurements within 120 minutes of sample dilution. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | At least 1 year at -20 degrees C |
| Product Form | Off-white amorphous powder |
| Activity / Unit Definition | One unit consumes 1 umol NADH per minute under the stated reaction conditions. |
| Molecular Weight | Approximately 50 kDa |
| pH Range / Optimal pH | Stable at pH 5.0-10.0; optimum pH 7.0-8.0 |
| Appearance | Off-white amorphous powder |
| Alias | Glutamate dehydrogenase (NAD dependent) |
| Product Type | NAD-dependent glutamate dehydrogenase enzyme raw material |
| Function / Efficacy | Consume NADH in the reductive amination reaction used for ammonia-related assays. |
| Abbreviation | GLDH |
| Customization | Specifications can be customized |
| Specific Activity | >=20 U/mg |
| Isoelectric Point | 5.92 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | Approximately 45 degrees C |
| Thermal Stability | Stable below 50 degrees C |
| Michaelis Constant | 10.4 x 10^-3 mol/L for NH4Cl; 7.42 x 10^-3 mol/L for alpha-ketoglutarate; 6.65 x 10^-4 mol/L for NADH |
| Detection Wavelength | 340 nm |
| Linear Range | 100-1,000 U/L; 100-500 U/L recommended |
For research use only, not for clinical use.
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