>=18 U/mg Lipoprotein Lipase for Triglyceride and Total Cholesterol Reagents

Name: >=18 U/mg Lipoprotein Lipase for Triglyceride and Total Cholesterol Reagents
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Cat.No: IEC-HMM-0229 Datasheet

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Product Name	>=18 U/mg Lipoprotein Lipase for Triglyceride and Total Cholesterol Reagents
Catalog No.	IEC-HMM-0229
Description	Alta DiagnoTech lipoprotein lipase is a light-brown lyophilized enzyme powder for glycerol-releasing triglyceride and total-cholesterol assay systems.
Intended Use	Enzyme raw material for research and bulk formulation of triglyceride and total-cholesterol reagents.
Principle / Technology	Coupled activity can be monitored from quinoneimine-dye formation at 555 nm.
Detection Method	Coupled spectrophotometric assay at 555 nm
Performance Range / Specifications	Specific activity >=18 U/mg dry powder.
Storage Conditions	Store and transport at -25 to -15 degrees C
Shelf Life	2 years at -25 to -15 degrees C
Product Form	Light-brown lyophilized powder
Activity / Unit Definition	One unit is the amount of enzyme that produces 1 umol glycerol per minute under the stated conditions.
Molecular Weight	30 kDa by SDS-PAGE
Source / Origin	Microbial source
pH Range / Optimal pH	Optimum pH 7.0-9.0; stable at pH 5.0-8.0 after 20 hours at 25 degrees C
Shipping Conditions	Transport at -25 to -15 degrees C
Recommended Buffer System	Use 0.1 M Britton-Robinson buffer for pH profiling and 20 mM potassium phosphate at pH 7.0 for temperature and thermal-stability studies; enzyme concentration 1 mg/mL.
Appearance	Light-brown lyophilized powder
Alias	Lipoprotein Lipase
Product Type	Lipoprotein lipase enzyme raw material
Function / Efficacy	Hydrolyze triglycerides to release glycerol for coupled biochemical detection.
Abbreviation	LPL
EC Number	EC 3.1.1.34
Specific Activity	>=18 U/mg dry powder
Isoelectric Point	5.9
Inhibitors	EDTA and SDS
Optimal Temperature	45-55 degrees C
Thermal Stability	Stable below 50 degrees C at pH 7.0 for 30 minutes
Stabilizer	BSA
Detection Wavelength	555 nm

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For research use only, not for clinical use.