>=18 U/mg Glycerol Kinase for 555 nm Triglyceride Assays

Name: >=18 U/mg Glycerol Kinase for 555 nm Triglyceride Assays
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Cat.No: IEC-HMM-0203 Datasheet

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Product Name	>=18 U/mg Glycerol Kinase for 555 nm Triglyceride Assays
Catalog No.	IEC-HMM-0203
Description	Alta DiagnoTech glycerol kinase is a white amorphous lyophilized enzyme powder for coupled triglyceride detection with glycerol-3-phosphate oxidase and lipoprotein lipase.
Intended Use	Used with glycerol-3-phosphate oxidase and lipoprotein lipase for triglyceride reagent research and bulk formulation.
Principle / Technology	Coupled activity is monitored from quinoneimine-dye formation at 555 nm.
Detection Method	Coupled spectrophotometric assay at 555 nm
Performance Range / Specifications	Dry-powder specific activity >=18 U/mg; electrophoretic purity >=90%.
Storage Conditions	Store at -25 to -15 degrees C
Shelf Life	18 months at -25 to -15 degrees C
Product Form	White amorphous lyophilized powder
Purity	>=90% electrophoretic purity
Molecular Weight	55 kDa by SDS-PAGE
Source / Origin	Microbial source
pH Range / Optimal pH	Optimum pH 7.5-8.5; stable at pH 4.5-10.0 for 16 hours at 25 degrees C
Recommended Buffer System	pH stability buffers: acetate at pH 4.5-5.5, phosphate at pH 6.0-8.0, Tris-HCl at pH 8.0-9.0 and glycine-NaOH at pH 9.0-10.0. Temperature and thermal-stability studies use 50 mM Tris-HCl at pH 8.0 and 1 mg/mL enzyme.
Appearance	White amorphous lyophilized powder
Alias	Glycerol Kinase
Product Type	Glycerol kinase enzyme raw material
Function / Efficacy	Phosphorylate glycerol in coupled triglyceride assay systems.
Abbreviation	GK
EC Number	EC 2.7.1.30
Specific Activity	>=18 U/mg dry powder
Optimal Temperature	50 degrees C
Thermal Stability	Stable below 65 degrees C
Michaelis Constant	3.7 x 10^-6 M for glycerol; 5.6 x 10^-5 M for ATP
Detection Wavelength	555 nm

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For research use only, not for clinical use.