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| Product Name | >=150 U/mg Lactate Dehydrogenase for 340 nm ALT/AST Assays |
| Catalog No. | IEC-HMM-0222 |
| Description | Alta DiagnoTech lactate dehydrogenase is a white amorphous enzyme powder characterized for NADH-based activity testing and use in alanine and aspartate aminotransferase reagent development. |
| Intended Use | Enzyme raw material for alanine aminotransferase and aspartate aminotransferase biochemical assay reagents. |
| Detection Method | Kinetic absorbance measurement at 340 nm |
| Performance Range / Specifications | Specific activity >=150 U/mg; assay linear range 100-1,000 U/L; recommended sample range 500-1,000 U/L. |
| Reaction Conditions / Protocol | Prepare Reagent 1 with 12.1 g Tris in 800 mL deionized water, adjust to pH 7.70 at 25 degrees C with HCl, dissolve 1 g sodium azide and bring to 1 L; immediately before use, add sodium pyruvate to 0.4 g/L. Prepare Reagent 2 with 21.1 g CAPS in 800 mL water, adjust to pH 9.70 with NaOH, dissolve 1.20 g NADH and 1 g sodium azide and bring to 1 L. For the assay, combine 1.00 mL Reagent 1 and 0.020 mL enzyme solution, preincubate for 60 seconds at 37 degrees C, add 0.200 mL Reagent 2, react for 1 minute, then determine the 1-minute absorbance-change rate at 340 nm. Activity in U/L equals Delta A/min x 9,807 x dilution factor. |
| Storage Conditions | Store at -20 degrees C; prepared assay reagents are stored at 2-8 degrees C. |
| Shelf Life | At least 1 year at -20 degrees C; prepared Reagent 1 and Reagent 2 are usable for 1 week at 2-8 degrees C. |
| Product Form | White amorphous powder |
| Activity / Unit Definition | One unit is the amount of enzyme that generates 1 umol NAD+ per minute under the stated reaction conditions. |
| Molecular Weight | 42 kDa |
| pH Range / Optimal pH | Stable at pH 4.5-10.0 for 24 hours at 25 degrees C; optimum pH 6.5-8.0 |
| Recommended Buffer System | Sample diluent: dissolve 1 g BSA and 1.36 g potassium dihydrogen phosphate in 800 mL deionized water, adjust to pH 7.0 at 25 degrees C with NaOH and bring to 1 L. The diluent is stable for 2 months at 2-8 degrees C. |
| Appearance | White amorphous powder |
| Alias | Lactate Dehydrogenase |
| Product Type | Lactate dehydrogenase enzyme raw material |
| Function / Efficacy | Provide lactate dehydrogenase activity for NADH-coupled aminotransferase assays. |
| Abbreviation | LDH |
| Customization | Specifications can be customized |
| Specific Activity | >=150 U/mg |
| Isoelectric Point | 4.98 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | 40-50 degrees C |
| Thermal Stability | No loss of activity below 55 degrees C for 15 minutes |
| Michaelis Constant | 4.0 x 10^-4 mol/L for pyruvate |
| Stabilizer | 0.1% BSA supports enzyme stability |
| Detection Wavelength | 340 nm |
| Linear Range | 100-1,000 U/L |
For research use only, not for clinical use.
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