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| Product Name | >=150 U/mg Glucose-6-Phosphate Dehydrogenase for 340 nm NADH Assays |
| Catalog No. | IEC-HMM-0197 |
| Description | Alta DiagnoTech glucose-6-phosphate dehydrogenase is a gray-white amorphous enzyme powder for glucose/hexokinase and creatine-kinase biochemical reagent development. |
| Intended Use | Enzyme raw material for glucose/hexokinase and creatine-kinase biochemical assay reagents. |
| Detection Method | Kinetic absorbance measurement of NADH formation at 340 nm |
| Performance Range / Specifications | Specific activity >=150 U/mg; recommended assay range 60-200 U/L. |
| Reaction Conditions / Protocol | Use 50 mM Tris-HCl at pH 7.8 containing 3.3 mM MgCl2, 60 mM NAD+ and 0.1 M glucose-6-phosphate. Combine 2.7 mL buffer, 0.1 mL NAD+ and 0.1 mL substrate, preincubate for 300 seconds at 30 degrees C, add 0.1 mL enzyme sample and determine the absorbance-change rate between minutes 4 and 5 at 340 nm. Dilute samples in 50 mM Tris-HCl at pH 7.5 with 0.1% BSA. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | At least 1 year at -20 degrees C |
| Product Form | Gray-white amorphous powder |
| Activity / Unit Definition | One unit is the amount of enzyme that converts 1 umol NAD+ to NADH per minute under the stated assay conditions. |
| Molecular Weight | Approximately 55 kDa |
| pH Range / Optimal pH | Stable at pH 5.0-10.5; optimum pH 8.5 |
| Appearance | Gray-white amorphous powder |
| Alias | Glucose-6-phosphate dehydrogenase |
| Product Type | Glucose-6-phosphate dehydrogenase enzyme raw material |
| Function / Efficacy | Catalyze NADH formation in a glucose-6-phosphate-dependent reaction. |
| Abbreviation | G6-P-DH |
| Customization | Specifications can be customized |
| Specific Activity | >=150 U/mg |
| Isoelectric Point | 4.8 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | >=55 degrees C |
| Thermal Stability | Stable below 50 degrees C |
| Michaelis Constant | 1.09 x 10^-4 M for glucose-6-phosphate; 1.4 x 10^-4 M for NAD+ |
| Detection Wavelength | 340 nm |
| Linear Range | 60-200 U/L |
For research use only, not for clinical use.
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